GreenMedInfo https://greenmedinfo.com en President Donald Trump signed an executive order to defund schools, universities and other education agencies that require COVID-19 vaccines for students and staff. Mon, 17 Feb 2025 20:49:16 +0000 314230 at https://greenmedinfo.com <p class="rtecenter"><img alt="" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/blank.justin/images/Breaking-Trump_to_Strip_Federal_Funding_From_Schools_That_Mandate_COVID_Vaccines-GreenMedInfo.jpg" style="width: 600px; height: 315px;" /><br /> <span style="font-size:12px;"><strong>Originally published on&nbsp;<a href="https://childrenshealthdefense.org/defender/trump-executive-order-defund-schools-covid-vaccine-mandates/" rel="dofollow" target="_blank">www.childrenshealthdefense.org</a>&nbsp;by&nbsp;Brenda Baletti, Ph.D.</strong></span></p> <p><span style="font-size:18px;"><em><strong>President Donald Trump signed an executive order to defund schools, universities and other education agencies that require COVID-19 vaccines for students and staff. The order directs HHS Secretary Robert F. Kennedy Jr. and the secretary of Education to create a plan to end the mandates.</strong></em></span></p><p><a href="https://greenmedinfo.com/content/breaking-trump-strip-federal-funding-schools-mandate-covid-vaccines" target="_blank">read more</a></p> Coronavirus Disease (Covid-19) Vaccination: All corruption in science government mass vaccination Mon, 17 Feb 2025 20:18:56 +0000 314227 at https://greenmedinfo.com PMID:  Iran J Basic Med Sci. 2024 ;27(12):1515-1528. PMID: 39539451 Abstract Title:  Anti-oxidant and anti-apoptotic effects of royal jelly against polystyrene microplastic-induced testicular injury in mice. Abstract:  OBJECTIVES: In recent years, microplastics (MPs), which are novel environmental contaminants measuring 5 mm in diameter, have garnered considerable attention. However, information regarding substances that can mitigate the dangers of MPs for animals remains extremely limited.MATERIALS AND METHODS: Ninety days were devoted to the exposure of mature male mice to royal jelly (RJ) and 2µm virgin polystyrene microplastics (PS-MPs) in this study. Pre-implantation embryo development; the structure of testis tissue; the gonadosomatic index; sperm parameters; RNA damage in germinal cells; the anti-oxidant capacity of the entire testis; and the activity of anti-oxidant enzymes in serum and testicular tissue, including TAC, SOD dismutase, CAT, GSH, and MDA, histomorphometric indices of the testis (tubular differentiation index, spermatogenesis index, and repopulation index), steroidogenic foci, and the quantity of apoptosis were assessed in the testis, respectively, through the measurement of pro-apoptosis (p53, Bax, and Caspase-3) and anti-apoptosis (Bcl-2) factors, as well as Hsp70 mediator.RESULTS: The results indicate that concurrent administration of RJ can confer a protective effect on mice exposed to microplastics by maintaining the structure of mitochondria and enhancement of the anti-oxidant defense system. Furthermore, RJ co-treatment decreased apoptosis and oxidant/anti-oxidant status, enhanced pre-implantation embryo development, and improved sperm characteristics and RNA damage in germ cells.CONCLUSION: The data confirm that royal jelly could protect the testis structure against polystyrene microplastic-induced testicular injury through anti-oxidant and anti-apoptotic properties. <p><a href="https://greenmedinfo.com/article/anti-oxidant-and-anti-apoptotic-effects-royal-jelly-against-polystyrene-microp" target="_blank">read more</a></p> Microplastic Toxicity Royal Jelly Testicular Injury: Chemical/Metal Induced Anti-Apoptotic Antioxidants Nano Plastic-and Micro-Particles Animal Study Thu, 13 Feb 2025 00:58:12 +0000 314202 at https://greenmedinfo.com <div class="copyright">This article is copyrighted by GreenMedInfo LLC, 2025<br/><strong><a href="/greenmedinfocom-re-post-guidelines">Visit our Re-post guidelines</a></strong></div><p>I recently posted an article on Facebook from Greenmedinfo without any censorship at&nbsp;https://www.facebook.com/GlyCop</p> <p>Please take a look and confirm it is being displayed.</p> censorship facebook Tue, 11 Feb 2025 03:15:30 +0000 314192 at https://greenmedinfo.com Why did U.S. Surgeon General Vivek Murthy push to censor true vaccine injury reports while accusing truth-tellers of 'killing people'? Mon, 10 Feb 2025 20:27:31 +0000 314186 at https://greenmedinfo.com <div class="copyright">This article is copyrighted by GreenMedInfo LLC, 2025<br/><strong><a href="/greenmedinfocom-re-post-guidelines">Visit our Re-post guidelines</a></strong></div><p class="rtecenter"><img alt="" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/blank.justin/images/02d2577e-2401-4454-a30d-716e7977f3d1_1082x728.jpg" /><br /> <span style="font-size:12px;"><strong>Originally published on&nbsp;<a href="https://sayerji.substack.com/p/truth-reconciliation-and-the-surgeon" rel="dofollow" target="_blank">www.sayerji.substack.com</a></strong></span></p> <p><span style="font-size:18px;"><em><strong><span style="letter-spacing: 0px;">Why did U.S. Surgeon General Vivek Murthy push to censor true vaccine injury reports while accusing truth-tellers of 'killing people'?</span></strong></em></span></p> <p><span style="font-size:18px;"><strong><span style="letter-spacing: 0px;">Who Was Really Spreading Misinformation?</span></strong></span></p> <p>The role of public health is to protect and inform the people. But what happens when the very institutions entrusted with that duty deliberately suppress truth and demonize those who seek to share it?</p><p><a href="https://greenmedinfo.com/content/truth-reconciliation-and-surgeon-generals-cover-vaccine-injuries" target="_blank">read more</a></p> Coronavirus Disease (Covid-19) Vaccination: All censorship corruption in science mass vaccination Mon, 10 Feb 2025 20:26:55 +0000 314183 at https://greenmedinfo.com Senate Bill 1437 would eliminate all non-medical exemptions as a way to ensure high rates of vaccination coverage. Fri, 07 Feb 2025 22:13:44 +0000 314169 at https://greenmedinfo.com <p class="rtecenter"><img alt="" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/blank.justin/images/Hawaii_Lawmakers_Float_Bill_to_Eliminate_Religious_Exemptions-GreenMedInfo.jpg" /><br /> <span style="font-size:12px;"><strong>Originally published on&nbsp;<a href="https://childrenshealthdefense.org/defender/hawaii-lawmakers-bill-eliminate-religious-exemptions/" rel="dofollow" target="_blank">www.childrenshealthdefense.org</a>&nbsp;by&nbsp;Brenda Baletti, Ph.D.</strong></span></p> <p><em style="font-size: 18px; letter-spacing: 0px;"><strong>Senate Bill 1437 would eliminate all non-medical exemptions as a way to ensure high rates of vaccination coverage. The bill is part of the "governor's package," a set of proposed bills that make up Gov. Josh Green's legislative agenda. Health freedom advocates said they expect significant public pushback.</strong></em></p><p><a href="https://greenmedinfo.com/content/hawaii-lawmakers-float-bill-eliminate-religious-exemptions" target="_blank">read more</a></p> Coronavirus Disease (Covid-19) Vaccination: All corruption in science government mass vaccination Fri, 07 Feb 2025 22:13:10 +0000 314168 at https://greenmedinfo.com Why is the Elevate Prize rewarding a figure accused of black operations, election meddling, and misinformation? Fri, 07 Feb 2025 20:04:05 +0000 314166 at https://greenmedinfo.com <div class="copyright">This article is copyrighted by GreenMedInfo LLC, 2025<br/><strong><a href="/greenmedinfocom-re-post-guidelines">Visit our Re-post guidelines</a></strong></div><p class="rtecenter"><img alt="" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/blank.justin/images/4885497b-46f8-44f6-9a91-37c8e745e665_1004x846.jpg" /><br /> <span style="font-size:12px;"><strong>Originally published on <a href="https://sayerji.substack.com/p/imran-ahmed-and-the-elevate-prize" rel="dofollow" target="_blank">www.sayerji.substack.com</a></strong></span></p> <p><em style="font-size: 18px; background-color: rgb(255, 255, 255); color: var(--print_secondary, var(--print_secondary_on_web_bg_color, var(--print_secondary, var(--color-fg-secondary)))); font-family: var(--font_family_headings, var(--font_family_headings_preset, var(--font-family-title)));"><strong>Why is the Elevate Prize rewarding a figure accused of black operations, election meddling, and misinformation?</strong></em></p><p><a href="https://greenmedinfo.com/content/imran-ahmed-and-elevate-prize-troubling-recognition-amid-controversy" target="_blank">read more</a></p> Vaccination: All censorship corruption in science technology Fri, 07 Feb 2025 20:03:38 +0000 314165 at https://greenmedinfo.com President Trump issued an executive order on January 20, 2025, for the United States to withdraw from the World Health Organization. Fri, 07 Feb 2025 19:27:00 +0000 314164 at https://greenmedinfo.com <p class="rtecenter"><img alt="" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/blank.justin/images/unnamed(8).jpg" style="width: 600px; height: 482px;" /><br /> <span style="font-size:12px;"><strong>Originally published on&nbsp;<a href="https://standforhealthfreedom.com/who-withdrawal/" rel="dofollow" target="_blank">www.standforhealthfreedom.com</a></strong></span></p><p><a href="https://greenmedinfo.com/content/who-withdrawal-analysis-and-next-steps" target="_blank">read more</a></p> Coronavirus Disease (Covid-19) government natural health politics Fri, 07 Feb 2025 19:26:03 +0000 314163 at https://greenmedinfo.com Exposed: U.S. taxpayer dollars are secretly funding a UK-based censorship network targeting American voices and interfering with our election integrity. Thu, 06 Feb 2025 19:43:22 +0000 314158 at https://greenmedinfo.com <div class="copyright">This article is copyrighted by GreenMedInfo LLC, 2025<br/><strong><a href="/greenmedinfocom-re-post-guidelines">Visit our Re-post guidelines</a></strong></div><p class="rtecenter"><img alt="" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/blank.justin/images/f63b1c1c-68bf-4119-8631-26104e75bd1a_1996x1248.jpg" /><br /> <span style="font-size:12px;"><strong><span style="letter-spacing: 0px;">Originally published on&nbsp;</span><a href="https://sayerji.substack.com/p/breaking-us-tax-dollars-secretly" rel="dofollow" target="_blank">www.sayerji.substack.com</a></strong></span></p> <p><em style="font-size: 18px; background-color: rgb(255, 255, 255); color: var(--print_secondary, var(--print_secondary_on_web_bg_color, var(--print_secondary, var(--color-fg-secondary)))); font-family: var(--font_family_headings, var(--font_family_headings_preset, var(--font-family-title)));"><strong>Exposed: U.S. taxpayer dollars are secretly funding a UK-based censorship network targeting American voices and interfering with our election integrity.</strong></em></p><p><a href="https://greenmedinfo.com/content/breaking-us-tax-dollars-secretly-fuel-ccdhs-dark-money-censorship-election-med" target="_blank">read more</a></p> Coronavirus Disease (Covid-19) big tech censorship free speech Thu, 06 Feb 2025 19:42:53 +0000 314157 at https://greenmedinfo.com Behind the veil of journalism and aid, a global censorship machine launders suppression under the guise of 'trusted news.' Wed, 05 Feb 2025 20:40:15 +0000 314150 at https://greenmedinfo.com <div class="copyright">This article is copyrighted by GreenMedInfo LLC, 2025<br/><strong><a href="/greenmedinfocom-re-post-guidelines">Visit our Re-post guidelines</a></strong></div><p class="rtecenter"><img alt="" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/blank.justin/images/b795a900-f7ec-43da-a98e-abe91c0ed2f8_728x520.jpg" /><br /> <span style="font-size:12px;"><strong>Originally published on&nbsp;<a href="https://sayerji.substack.com/p/laundering-censorship-the-bbc-usaid" rel="dofollow" target="_blank">www.sayerji.substack.com</a></strong></span></p> <p><span style="font-size:18px;"><em><strong><span style="letter-spacing: 0px;">Behind the veil of journalism and aid, a global censorship machine launders suppression under the guise of 'trusted news.'</span></strong></em></span></p><p><a href="https://greenmedinfo.com/content/usaid-bbc-caught-laundering-censorship-unconstitutional-unforgivable" target="_blank">read more</a></p> Coronavirus Disease (Covid-19) Vaccination: All corruption in science free speech technology Wed, 05 Feb 2025 20:38:26 +0000 314147 at https://greenmedinfo.com In a disturbing revelation published in Nature Medicine, scientists find that the average human brain now harboring approximately 7 grams of microscopic plastic particles. Tue, 04 Feb 2025 18:20:54 +0000 314135 at https://greenmedinfo.com <div class="copyright">This article is copyrighted by GreenMedInfo LLC, 2025<br/><strong><a href="/greenmedinfocom-re-post-guidelines">Visit our Re-post guidelines</a></strong></div><p class="rtecenter"><img alt="" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/blank.justin/images/Shocking_Discovery-Human_Brain_Cntains_7_Grams_of_Microplastics_50_More_Than_Previous_Estimates-GreenMedInfo.jpg" style="width: 600px; height: 315px;" /></p> <p><em style="letter-spacing: 0px;">Picture a sugar packet - that's roughly how much microplastic is now accumulating in the average human brain, according to groundbreaking new research from the University of New Mexico. In a disturbing revelation published in Nature Medicine, scientists have documented a 50% increase in brain microplastic concentrations over just eight years, with the average human brain now harboring approximately 7 grams of microscopic plastic particles. To put this in perspective, that's about the weight of seven paper clips worth of plastic fragments dispersed throughout our neural tissue.</em></p><p><a href="https://greenmedinfo.com/content/shocking-discovery-human-brain-contains-7-grams-microplastics-50-more-previous" target="_blank">read more</a></p> Dementia Microplastic Toxicity brain health microplastics pollution Tue, 04 Feb 2025 18:19:42 +0000 314133 at https://greenmedinfo.com PMID:  Drug Dev Ind Pharm. 2020 Sep ;46(9):1550-1557. Epub 2020 Aug 27. PMID: 32811191 Abstract Title:  Antihyperuricemic efficacy of Scopoletin-loaded Soluplus micelles in yeast extract/potassium oxonate-induced hyperuricemic mice. Abstract:  Scopoletin (Sco) has great potential for hyperuricemia therapy. However, the relatively low oral bioavailability of Sco limits its further applications. Soluplus-based Sco micelles (Sco-Ms) were successfully prepared in our previous work. The oral bioavailability of Sco-Ms was increased by 438% compared with free Sco. In this study, we aimed to compare the biodistribution and antihyperuricemic efficacy of Sco and Sco-Ms, and explore their therapeutic mechanisms as well. We studied the tissue biodistribution of Sco and Sco-Ms after they were orally administered to mice. The antihyperuricemic effect and the therapeutic mechanisms of Sco and Sco-Ms were evaluated using yeast extract/potassium oxonate-induced hyperuricemia model in mice. The Sco concentration in each tissue was significantly higher than that of Sco suspension after orally administrating Sco-Ms to mice. Oral delivery of Sco-Ms exhibited significantly stronger hypouricemic efficacy in hyperuricemic mice than Sco. Meanwhile, Sco-Ms showed a better protective effect on mice kidney injury. The hypouricemic efficacy of Sco was due to promoting the excretion of uric acid via modulating the alteration of gene expression levels of renal uric acid transporter (URAT1), glucose transporter (GLUT9), and organic anion transporter 1 (OAT1). Sco-Ms could not only restore the dysregulation of URAT1, GLUT9, and OAT1 more effectively, but also down-regulate the activity of hepatic xanthine oxidase (XOD) to inhibit the production of uric acid. In conclusion, taken together, Sco-Ms represents a potential oral strategy for the treatment of hyperuricemia. <p><a href="https://greenmedinfo.com/article/antihyperuricemic-efficacy-scopoletin-loaded-soluplus-micelles-yeast-extractpo" target="_blank">read more</a></p> Hyperuricemia Scopoletin Uricosuric Agents Animal Study Tue, 04 Feb 2025 00:13:02 +0000 314125 at https://greenmedinfo.com PMID:  Int Immunopharmacol. 2013 Dec ;17(4):1037-43. PMID: 24455774 Abstract Title:  Scopoletin suppresses IL-6 production from fibroblast-like synoviocytes of adjuvant arthritis rats induced by IL-1βstimulation. Abstract:  Scopoletin, a coumarin compound naturally occurring in many medicinal plants, has previously been demonstrated to ameliorate synovial inflammation and destruction of cartilage and bone in adjuvant arthritis (AA) rats. As interleukin (IL)-6 is critically involved in the initiation and development of rheumatoid arthritis (RA), the present study was performed to investigate the effect of scopoletin on IL-6 production from fibroblast-like synoviocytes (FLS) to get insight into its anti-RA mechanisms. FLS were isolated from synovial membrane tissues of AA rats, and stimulated with IL-1β(10 ng/mL). Scopoletin, at concentrations of 15, 30, and 60μM, was shown to only moderately inhibit FLS proliferation, but dramatically reduce IL-6 production at both mRNA and protein levels. It also inhibited the phosphorylation of p38 mitogen-activated protein kinase, extracellular signal-regulated kinase (ERK), protein kinase C (PKC) and cAMP response element binding protein (CREB). These findings suggest that scopoletin exerts anti-RA action probably through suppressing IL-6 production from FLS via MAPK/PKC/CREB pathways. <p><a href="https://greenmedinfo.com/article/scopoletin-suppresses-il-6-production-fibroblast-synoviocytes-adjuvant-arthrit" target="_blank">read more</a></p> Arthritis Scopoletin Anti-Inflammatory Agents Interleukin-1 beta downregulation Interleukin-6 Downregulation Animal Study Tue, 04 Feb 2025 00:10:11 +0000 314123 at https://greenmedinfo.com PMID:  Eur J Pharmacol. 2018 Sep 5 ;834:152-156. Epub 2018 Jul 19. PMID: 30031794 Abstract Title:  Scopoletin inhibitsα-glucosidase in vitro and alleviates postprandial hyperglycemia in mice with diabetes. Abstract:  The aim of this study was to investigate whether scopoletin could inhibit the activities of the carbohydrate digestive enzymes,α-glucosidase andα-amylase, and reduce postprandial hyperglycemia in streptozotocin (STZ)-induced diabetes in mice. Scopoletin showed a distinct inhibitory effect onα-glucosidase andα-amylase. The half maximal inhibitory concentration (IC) of scopoletin was 85.12 and 37.36 μM forα-glucosidase andα-amylase, respectively, which were lower values than those for acarbose. The increase in postprandial blood glucose levels was significantly suppressed in the scopoletin group compared to the control group of STZ-induced diabetes in mice. Moreover, the area under the curve significantly decreased with the administration of scopoletin in STZ-induced diabetes in mice. These results showed that scopoletin might help to lower postprandial hyperglycemia through inhibition of carbohydrate digestive enzymes. <p><a href="https://greenmedinfo.com/article/scopoletin-inhibits-glucosidase-vitro-and-alleviates-postprandial-hyperglycemi" target="_blank">read more</a></p> Hyperglycemia Scopoletin Alpha-glucosidase inhibitor Animal Study Mon, 03 Feb 2025 22:22:34 +0000 314120 at https://greenmedinfo.com PMID:  Int J Mol Med. 2018 Oct ;42(4):2285-2293. Epub 2018 Jul 4. PMID: 30015831 Abstract Title:  Scopoletin downregulates MMP‑1 expression in human fibroblasts via inhibition of p38 phosphorylation. Abstract:  Irradiation of keratinocytes by ultraviolet B induces cytokine production, which in turn activates fibroblasts to produce cytokines and increase matrix metallopeptidase (MMP)‑1 protein expression. The present study investigated the effect and potential mechanisms of scopoletin on the regulation of MMP‑1 expression in fibroblasts. Scopoletin was isolated from Artemisia capillaris crude extract. Treatment of fibroblasts with scopoletin resulted in a decrease in the protein expression of MMP‑1 following stimulation with human keratinocyte (HaCaT) conditioned medium. To further explore the mechanism underlying this effect, the expression levels of proteins in the mitogen‑activated protein kinase (MAPK) and nuclear factor‑κB (NF‑κB) signaling pathways were evaluated via western blot analysis. The mRNA expression levels of interleukin (IL)‑1αand tumor necrosis factor (TNF)αwere evaluated via reverse transcription‑quantitative polymerase chain reaction. The effect of scopoletin on cell viability was assessed with the MTT assay. The results demonstrated that scopoletin treatment markedly decreased MMP‑1, IL‑1αand TNFαmRNA expression in fibroblasts stimulated with HaCaT conditioned medium (40 mJ/cm2), without any apparent cell cytotoxicity, and in a dose‑dependent manner. In addition, western blot analysis demonstrated that scopoletin reduced the phosphorylation of p38 MAPK in fibroblasts. In summary, the present study demonstrated that scopoletin inhibited MMP‑1 and proinflammatory cytokine expression by inhibiting p38 MAPK phosphorylation. These findings suggest that scopoletin may have potential as a therapeutic agent to prevent and treat photoaging of the skin. <p><a href="https://greenmedinfo.com/article/scopoletin-downregulates-mmp-1-expression-human-fibroblasts-inhibition-p38-pho" target="_blank">read more</a></p> Aging Skin Scopoletin Anti-Inflammatory Agents Interleukin-1 alpha downregulation Matrix metalloproteinase-1 (MMP-1) inhibitor Tumor Necrosis Factor (TNF) Alpha Inhibitor In Vitro Study Mon, 03 Feb 2025 22:20:09 +0000 314117 at https://greenmedinfo.com PMID:  Life Sci. 2005 Jul 1 ;77(7):824-36. Epub 2005 Apr 7. PMID: 15936354 Abstract Title:  Scopoletin induces apoptosis in human promyeloleukemic cells, accompanied by activations of nuclear factor kappaB and caspase-3. Abstract:  Scopoletin (6-methoxy-7-hydroxycoumarin) is a phenolic coumarin and a member of the phytoalexins. In this study we investigated whether scopoletin caused apoptosis in HL-60 promyelocytic cells and, if so, by what mechanisms. We found that scopoletin induced apoptosis as confirmed by a characteristic ladder pattern of discontinuous DNA fragments in a dose-dependent manner. The signal cascade activated by scopoletin included the heterodimeric redox-sensitive transcription factor NF-kappaB, which exhibited an upregulation of nuclear factor-kappa B (NF-kappaB) translocation to the nucleus by increase of IkappaBalpha degradation. In addition, scopoletin activated caspase-3 as was evidenced by both the proteolytic cleavage of the proenzyme and increased protease activity. Activation of caspase-3 resulted in the cleavage of 116 kDa poly(ADP-ribose) polymerase (PARP) to 85 kDa cleavage product in time-and dose-dependent fashions. Prior treatment of the cells with pyrrolidine dithiocarbamate, a potent inhibitor of NF-kappaB activation, or Ac-DEVD-CHO, a specific caspase-3 inhibitor, prevented scopoletin-induced caspase-3 activation, PARP cleavage, and finally DNA fragmentation. Taken together, these results suggest that scopoletin induces NF-kappaB activation, which, in turn, causes activation of caspase-3, degradation of PARP, and eventually leads to apoptotic cell death in HL-60 cells. <p><a href="https://greenmedinfo.com/article/scopoletin-induces-apoptosis-human-promyeloleukemic-cells" target="_blank">read more</a></p> Promyelocytic leukemia Scopoletin Apoptotic NF-kappaB Inhibitor In Vitro Study Mon, 03 Feb 2025 21:57:29 +0000 314114 at https://greenmedinfo.com PMID:  Food Funct. 2012 Aug ;3(8):886-90. Epub 2012 Jun 27. PMID: 22733205 Abstract Title:  Scopoletin attenuates allergy by inhibiting Th2 cytokines production in EL-4 T cells. Abstract:  Scopoletin is an antioxidant found in certain weedy plants. It exerts anti-inflammatory, anti-allergic, and anti-diabetic activities. It remains unknown whether scopoletin regulates T helper (Th) cells, including Th 1 and Th 2 cells. We found that scopoletin significantly inhibited phorbol myristate acetate (PMA)/ionomycin-induced interleukin-4 (IL-4), IL-5, and IL-10 production in EL-4 T cells. In addition, scopoletin significantly enhanced the inhibitory action of PMA/ionomycin on interferon-γ(IFN-γ) expression. In EL-4 T cells, PMA/ionomycin treatment markedly increased the expression of nuclear factor of activated T cells (NFAT) and GATA-3; in contrast, scopoletin significantly down-regulated expressions of these transcription factors. Furthermore, this downregulation depended on protein kinase C (PKC) attenuation. This leads us to suggest that the anti-allergic properties of scopoletin might be mediated by the downregulation of cytokine expression in Th 2 cells. <p><a href="https://greenmedinfo.com/article/scopoletin-attenuates-allergy-inhibiting-th2-cytokines-production-el-4-t-cells" target="_blank">read more</a></p> Allergies Scopoletin Anti-Allergic Agents Immunomodulatory Phytotherapy In Vitro Study Mon, 03 Feb 2025 21:24:50 +0000 314113 at https://greenmedinfo.com PMID:  Metabolism. 2014 Apr ;63(4):593-601. Epub 2014 Jan 16. PMID: 24559844 Abstract Title:  Scopoletin prevents alcohol-induced hepatic lipid accumulation by modulating the AMPK-SREBP pathway in diet-induced obese mice. Abstract:  OBJECTIVE: This study investigated the effects of scopoletin on alcohol-induced hepatic lipid accumulation in diet-induced obese mice and its mechanism.MATERIAL/METHODS: Alcohol (25% v/v, 5g/kg body weight) was orally administered once a day for 6 weeks to mice fed with a high-fat diet (35%kcal) with or without scopoletin (0.05%, wt/wt).RESULTS: Scopoletin reduced plasma acetaldehyde, fatty acid, total cholesterol, triglyceride and insulin levels, hepatic lipid and droplets and fasting blood glucose levels that were increased by alcohol. Scopoletin significantly activated hepatic AMPK and inhibited ACC and SREBP-1c and the activities of lipogenic enzymes, such as FAS, PAP and G6PD compared to the alcohol control group. Moreover, scopoletin significantly inhibited hepatic CYP2E1 activity and protein levels but elevated the activities of SOD, CAT, GSH-Px and GST and the levels of GSH compared to the alcohol control group. The hepatic lipid peroxide level was significantly lowered by scopoletin supplementation in alcohol-administered obese mice.CONCLUSIONS: Taken together, these results suggested that scopoletin can ameliorate alcohol-induced hepatic lipid accumulation by modulating AMPK-SREBP pathway-mediated lipogenesis in mice fed a high-fat diet. <p><a href="https://greenmedinfo.com/article/scopoletin-prevents-alcohol-induced-hepatic-lipid-accumulation-modulating-ampk" target="_blank">read more</a></p> Alcohol Toxicity Lipid Peroxidation Obesity Scopoletin Hepatoprotective Hypolipidemic Animal Study Mon, 03 Feb 2025 21:05:01 +0000 314110 at https://greenmedinfo.com n/a PMID:  Int Immunopharmacol. 2022 Jan ;102:108382. Epub 2021 Nov 28. PMID: 34848155 Abstract Title:  Ameliorate impacts of scopoletin against vancomycin-induced intoxication in rat model through modulation of Keap1-Nrf2/HO-1 and IκBα-P65 NF-κB/P38 MAPK signaling pathways: Molecular study, molecular docking evidence and network pharmacology analysis. Abstract:  Nephrotoxicity is an indication for the damage of kidney-specific detoxification and excretion mechanisms by exogenous or endogenous toxicants. Exposure to vancomycin predominantly results in renal damage and losing the control of body homeostasis. Vancomycin-treated rats (200 mg/kg/once daily, for seven consecutive days, i.p.) revealed significant increase in serum pivotal kidney function, oxidative stress, and inflammatory biomarkers. Histologically, vancomycin showed diffuse acute tubular necrosis, denudation of epithelium and infiltration of inflammatory cells in the lining tubular epithelium in cortical portion. In the existing study, the conservative consequences of scopoletin against vancomycin nephrotoxicity was investigated centering on its capacity to alleviate oxidative strain and inflammation through streamlining nuclear factor (erythroid-derived-2) like 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling and prohibiting the nuclear factor kappa B (NF-κB)/mitogen-activated protein kinase (p38 MAPK) pathway. With respect to vancomycin group, scopoletin pretreatment (50 mg/kg/once daily, i.p.) efficiently reduced kidney function, oxidative stress biomarkers and inflammatory mediators. Moreover, histological and immunohistochemical examination of scopoletin-treated group showed remarkable improvement in histological structure and reduced vancomycin-induced renal expression of iNOS, NF-κB and p38 MAPK. In addition, scopoletin downregulated (Kelch Like ECH Associated Protein1) Keap1, P38MAPK and NF-κB expression levels while upregulated renal expression levels of regulatory protein (IκBα), Nrf2 and HO-1. Furthermore, molecular docking and network approach were constructed to study the prospect interaction between scopoletin and the targeted proteins that streamline oxidative stress and inflammatory pathways. The present investigations elucidated that scopoletin co-treatment with vancomycin may be a rational curative protocol for mitigation of vancomycin-induced renal intoxication. Kidney Damage: Drug-Induced Scopoletin Antioxidants Heme oxygenase-1 up-regulation Nrf2 activation Renoprotective Vancomycin Drug: Vancomycin Animal Study Mon, 03 Feb 2025 21:02:11 +0000 314107 at https://greenmedinfo.com During Thursday's U.S. Senate confirmation hearing for RFK Jr., Sen. Cassidy cited a meta-analysis published in 2014 in the journal Vaccine as scientific proof that vaccines don’t cause autism. Mon, 03 Feb 2025 20:06:52 +0000 314104 at https://greenmedinfo.com <p class="rtecenter"><img alt="" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/blank.justin/images/Sen__Cassidy_Told_RFK_Jr_This_Study_Proves_Vaccines_Dont_Cause_Autism-Heres_Whats_Wrong_With_the_Study-GreenMedInfo.jpg" /><br /> <span style="font-size:12px;"><strong>Originally published on&nbsp;<a href="https://tdefender.substack.com/p/bill-cassidy-meta-analysis-vaccines-autism-rfk-jr-senate-hearing" rel="dofollow" target="_blank">www.tdefender.substack.com</a>&nbsp;by&nbsp;Brian Hooker, Ph.D.</strong></span></p> <p><span style="font-size:18px;"><em><strong><span style="letter-spacing: 0px;">During Thursday's U.S. Senate confirmation hearing for RFK Jr., Sen. Cassidy cited a meta-analysis published in 2014 in the journal Vaccine as scientific proof that vaccines don't cause autism.</span></strong></em></span></p><p><a href="https://greenmedinfo.com/content/sen-cassidy-told-rfk-jr-study-proves-vaccines-dont-cause-autism-heres-what-s-w" target="_blank">read more</a></p> Autism Spectrum Disorders Diphtheria Measles Mumps Pertussis Rubella Tetanus Vaccination: All autism spectrum disorders corruption in science mass vaccination Mon, 03 Feb 2025 20:06:09 +0000 314103 at https://greenmedinfo.com PMID:  Mol Biol Rep. 2024 May 6 ;51(1):620. Epub 2024 May 6. PMID: 38709349 Abstract Title:  Scopoletin alleviates high glucose-induced toxicity in human renal proximal tubular cells via inhibition of oxidative damage, epithelial-mesenchymal transition, and fibrogenesis. Abstract:  BACKGROUND: Recent years of evidence suggest the crucial role of renal tubular cells in developing diabetic kidney disease. Scopoletin (SCOP) is a plant-based coumarin with numerous biological activities. This study aimed to determine the effect of SCOP on renal tubular cells in developing diabetic kidney disease and to elucidate mechanisms.METHODS AND RESULTS: In this study, SCOP was evaluated in vitro using renal proximal tubular (HK-2) cells under hyperglycemic conditions to understand its mechanism of action. In HK-2 cells, SCOP alleviated the high glucose-generated reactive oxygen species (ROS), restored the levels of reduced glutathione, and decreased lipid peroxidation. High glucose-induced alteration in the mitochondrial membrane potential was markedly restored in the SCOP-treated cells. Moreover, SCOP significantly reduced the high glucose-induced apoptotic cell population in the Annexin V-FITC flow cytometry study. Furthermore, high glucose markedly elevated the mRNA expression of fibrotic and extracellular matrix (ECM) components, namely, transforming growth factor (TGF)-β, alfa-smooth muscle actin (α-SMA), collagen I, and collagen III, in HK-2 cells compared to the untreated cells. SCOP treatment reduced these mRNA expressions compared to the high glucose-treated cells. Collagen I and TGF-βprotein levels were also significantly reduced in the SCOP-treated cells. Further findings in HK-2 cells revealed that SCOP interfered with the epithelial-mesenchymal transition (EMT) in the high glucose-treated HK-2 cells by normalizing E-cadherin and downregulating the vimentin andα-SMA proteins.CONCLUSIONS: In conclusion, SCOP modulates the high glucose-generated renal tubular cell oxidative damage and accumulation of ECM components and may be a promising molecule against diabetic nephropathy. <p><a href="https://greenmedinfo.com/article/scopoletin-alleviates-high-glucose-induced-toxicity-human-renal-proximal-tubul" target="_blank">read more</a></p> Diabetic Nephropathy Lipid Peroxidation Oxidative Stress Scopoletin Anti-Apoptotic Antioxidants Renoprotective In Vitro Study Mon, 03 Feb 2025 04:09:29 +0000 314097 at https://greenmedinfo.com PMID:  Phytomedicine. 2015 Mar 15 ;22(3):362-8. Epub 2015 Feb 4. PMID: 25837273 Abstract Title:  Scopoletin has a potential activity for anti-aging via autophagy in human lung fibroblasts. Abstract:  Autophagy was known to be associated with aging in addition to cancer and neurodegeneration. The effects of scopoletin on autophagy and anti-aging were investigated in human lung fibroblast cell line, IMR 90. Here we show that scopoletin induces autophagy. It is also identified that the modulation of p53 by scopoletin are related to the induction of autophagy. Moreover, the level of SA-β-Gal staining, an aging marker, is reduced by scopoletin. In addition, while the expression levels of histone deacetylases such as HDAC1, SIRT1 and SIRT6 are increased in IMR 90 cells in the presence of scopoletin, the expression levels of histone acetyltransferases are decreased. Furthermore, scopoletin enhances the level of transcription factors such as Nrf-2and p-FoxO1 related to anti-aging. In addition, scopoletin modulates the reprogramming proteins. Therefore, these findings suggest that scopoletin could exert a positive effect on anti-aging related to autophagy through modulation of p53 in human lung fibroblasts. <p><a href="https://greenmedinfo.com/article/scopoletin-has-potential-activity-anti-aging-autophagy-human-lung-fibroblasts" target="_blank">read more</a></p> Aging Scopoletin Antioxidants Nrf2 activation In Vitro Study Mon, 03 Feb 2025 02:41:07 +0000 314094 at https://greenmedinfo.com