Madin Darby Canine Kidney Cell line (MDCK) https://greenmedinfo.com/category/keywords/Madin%20Darby%20Canine%20Kidney%20Cell%20line%20%28MDCK%29 en In vitro and in vivo growth alter the population dynamic and properties of a Jeryl Lynn mumps vaccine. https://greenmedinfo.com/article/vitro-and-vivo-growth-alter-population-dynamic-and-properties-jeryl-lynn-mumps PMID:  Vaccine. 2015 Aug 26 ;33(36):4586-93. Epub 2015 Jul 15. PMID: 26187256 Abstract Title:  In vitro and in vivo growth alter the population dynamic and properties of a Jeryl Lynn mumps vaccine. Abstract:  Mumps vaccines are live attenuated viruses. They are known to vary in effectiveness, degree of attenuation and adverse event profile. However, the underlying reasons are poorly understood. We studied two closely related mumps vaccines which originate from the same attenuated Jeryl Lynn-5 strain but have different efficacies. Jeryl Lynn-Canine Kidney (JL-CK), produced on primary canine kidney cells, is less effective than RIT4385, which is produced on chicken embryo fibroblasts. JL-CK and RIT4385 could be distinguished by a number of in vitro and in vivo properties. JL-CK produced heterogeneous, generally smaller plaques than RIT4385, but gave 100-fold higher titres when grown in cells and showed a higher degree of hydrocephalus formation in neonatal rat brains. Sanger sequencing of JL-CK identified 14 regions of heterogeneity throughout the genome. Plaque purification of JL-CK demonstrated the presence of five different Jeryl Lynn-5 variants encompassing the 14 mutations. One JL-CK mutation was associated with a small plaque phenotype, the effects of the others in vitro or in vivo were less clear. Only 4% of the JL-CK population corresponded to the parental Jeryl Lynn-5 strain. Next generation sequencing of JL-CK and virus before and after growth in cell lines or neonatal rat brains showed that propagation in vitro or in vivo altered the population dramatically. Our findings indicate that growth of JL-CK in primary canine kidney cells resulted in the selection of a mixture of mumps virus variants that have different biological properties compared to the parent Jeryl Lynn-5 virus. We also report three previously unknown heterogenic regions within the N gene of the RIT4385 vaccine. <p><a href="https://greenmedinfo.com/article/vitro-and-vivo-growth-alter-population-dynamic-and-properties-jeryl-lynn-mumps" target="_blank">read more</a></p> https://greenmedinfo.com/article/vitro-and-vivo-growth-alter-population-dynamic-and-properties-jeryl-lynn-mumps#comments Mumps Vaccination: Mumps Vaccination: Mumps-Measles-Rubella (MMR) Madin Darby Canine Kidney Cell line (MDCK) Animal Study In Vitro Study Sun, 03 Mar 2019 20:55:43 +0000 greenmedinfo 180637 at https://greenmedinfo.com Suspensions of these cells injected intravenously in the chick embryo produce brain metastases resembling adenocarcinoma. https://greenmedinfo.com/article/suspensions-these-cells-injected-intravenously-chick-embryo-produce-brain-meta PMID:  Science. 1969 Jan 31 ;163(3866):472-3. PMID: 5762397 Abstract Title:  Secretory activity and oncogenicity of a cell line (MDCK) derived from canine kidney. Abstract:  A cell line (MDCK) of dog kidney origin grows on a glass surface as a mosaic of epithelium with many multicellular hemispherical vesicles. The cells lining the blisters actively secrete into the cyst cavities. Suspensions of these cells injected intravenously in the chick embryo produce brain metastases resembling adenocarcinoma. <p><a href="https://greenmedinfo.com/article/suspensions-these-cells-injected-intravenously-chick-embryo-produce-brain-meta" target="_blank">read more</a></p> https://greenmedinfo.com/article/suspensions-these-cells-injected-intravenously-chick-embryo-produce-brain-meta#comments Vaccination: All Madin Darby Canine Kidney Cell line (MDCK) In Vitro Study Sun, 29 Jul 2018 19:32:25 +0000 greenmedinfo 168158 at https://greenmedinfo.com