Ischemia: Myocardial https://greenmedinfo.com/category/keywords/Ischemia%3A%20Myocardial en Protective effects of carnosic acid against mitochondria-mediated injury in H9c2 cardiomyocytes induced by hypoxia/reoxygenation. https://greenmedinfo.com/article/protective-effects-carnosic-acid-against-mitochondria-mediated-injury-h9c2-car n/a PMID:  Exp Ther Med. 2017 Dec ;14(6):5629-5634. Epub 2017 Oct 2. PMID: 29285102 Abstract Title:  Protective effects of carnosic acid against mitochondria-mediated injury in H9c2 cardiomyocytes induced by hypoxia/reoxygenation. Abstract:  Myocardial ischemia and reperfusion occurs in myocardial infarction. Timely reperfusion will exacerbate the injury through the mitochondria-mediated apoptosis in cardiomyocytes due to the accumulation of excessive reactive oxygen species (ROS). In order to identify novel therapeutic approaches, the cardioprotective effects of carnosic acid and the underlying mechanisms were investigated in the present study in H9c2 cardiomyocytes injured by hypoxia/reoxygenation in vitro. The viability of H9c2 cardiomyocytes was detected by MTT assay and further confirmed by the detection of intracellular lactate dehydrogenase (LDH) release. The mitochondrial function in H9c2 cardiomyocytes was evaluated using fluorescence methods. The proteins related to apoptosis, including caspase-3, B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax) were analyzed by western blot analysis, and the activity of caspase-3 was determined using a colorimetric method. As a result, carnosic acid was demonstrated to improve the viability of H9c2 cardiomyocytes and suppress the leakage of cytosolic lactate dehydrogenase under hypoxia/reoxygenation. In addition, the overproduction of intracellular ROS and intracellular calcium overload were ameliorated in the presence of carnosic acid. The dysfunction of mitochondria in H9c2 cardiomyocytes was also attenuated by carnosic acid through blocking the collapse of mitochondrial membrane potential (MMP) and mitochondrial permeability transition pore (mPTP) opening. Furthermore, the apoptosis of H9c2 cardiomyocytes resulted from hypoxia/reoxygenation was inhibited by carnosic acid through the upregulation of Bcl-2 and the downregulation of Bax and caspase-3 levels. These results provided evidence for further investigation that would assist in the development of novel therapeutic approaches for myocardial infarction. https://greenmedinfo.com/article/protective-effects-carnosic-acid-against-mitochondria-mediated-injury-h9c2-car#comments Carnosic Acid Hypoxia Ischemia: Myocardial Cardioprotective Cardioprotective Carnosic Acid Hypoxia Ischemia: Myocardial In Vitro Study Tue, 09 Jan 2018 21:45:15 +0000 greenmedinfo 158267 at https://greenmedinfo.com V. album leaf extracts can mediate nitric oxide-dependent cardioprotection against myocardial injury produced by ischemia/reperfusion insult. https://greenmedinfo.com/article/v-album-leaf-extracts-can-mediate-nitric-oxide-dependent-cardioprotection-agai n/a PMID:  J Ethnopharmacol. 2017 Jul 6 ;209:203-209. Epub 2017 Jul 6. PMID: 28689799 Abstract Title:  Cardioprotective effects of Viscum album L. subsp. album (European misletoe) leaf extracts in myocardial ischemia and reperfusion. Abstract:  ETHNOPHARMACOLOGICAL RELEVANCE: Viscum album L. (European mistletoe) is a hemiparasitic plant belonging to Loranthaceae family and has been used in Turkish traditional medicine for the treatment of cardiovascular disorders and heart diseases such as hypertension, tachycardia and angina pectoris. AIM OF THE STUDY: The present study investigated the cardioprotective effects of V. album leaf extracts in myocardial ischemia and reperfusion injury in rats. MATERIAL AND METHODS: Lyophilized aqueous (AVa) and methanolic (MVa) extracts of V. album were prepared from dried leaf. The isolated hearts were perfused with V. album extracts prior to and during 35min of ischemia induced by coronary artery occlusion. After 120min of coronary reperfusion, infarct size was determined by triphenyltetrazolium staining. RESULTS: Both AVa and MVa extracts reduced the extent of infarction compared with untreated control hearts, but protective effect of MVa had more potential in low concentration; infarct size as proportion of ischemic risk zone: AVa 17.5±1.5%; Mva 20.3±2.5%, both P<0.01 versus control 38.1±1.4%. This protective effect was comparable to infarct limitation induced by ischemic preconditioning (21.5±2.4%). Inhibition of nitric oxide synthesis with L-N(G)-nitroarginine methyl ester completely abrogated the protection afforded by both extracts. ATP-sensitive K(+) channel blockade by glibenclamide abrogated the protection afforded by MVa while attenuating, but not abolishing, the protective action of Ava. CONCLUSIONS: This study provided the first experimental evidence that V. album leaf extracts can mediate nitric oxide-dependent cardioprotection against myocardial injury produced by ischemia/reperfusion insult. With this study, popular usage of V. album extracts in Turkish folk medicine as a remedy for cardiac diseases was justified. https://greenmedinfo.com/article/v-album-leaf-extracts-can-mediate-nitric-oxide-dependent-cardioprotection-agai#comments Ischemia: Myocardial Mistletoe Cardioprotective Cardioprotective Ischemia: Myocardial Mistletoe Plant Extracts Animal Study Thu, 17 Aug 2017 22:39:14 +0000 greenmedinfo 151826 at https://greenmedinfo.com