Anti-Bacterial Agents https://greenmedinfo.com/category/keywords/anti-bacterial-agents en Abscisic acid isolated from Korean acacia honey can be therapeutic for H. pylori-induced infections. https://greenmedinfo.com/article/abscisic-acid-isolated-korean-acacia-honey-can-be-therapeutic-h-pylori-induced n/a PMID:  Pharmacogn Mag. 2017 Jul ;13(Suppl 2):S170-S173. Epub 2017 Jul 11. PMID: 28808376 Abstract Title:  Isolation of Abscisic Acid from Korean Acacia Honey with Anti-Helicobacter pylori Activity. Abstract:  BACKGROUND: Helicobacter pylori (H. pylori) is linked to the development of the majority of peptic ulcers and some types of gastric cancers, and its antibiotic resistance is currently found worldwide. OBJECTIVE: This study is aimed at evaluating the anti-H. pylori activity of Korean acacia honey and isolating the related active components using organic solvents. MATERIAL AND METHODS: The crude acacia honey was extracted with n-hexane, dichloromethane, ethyl acetate (EtOAc), and n-butanol. The EtOAc extract was subjected to octadecyl-silica chromatography. The extracts and fractions were then examined for anti-H. pylori activity using the agar well diffusion method. The antimicrobial activity of abscisic acid against H. pylori was investigated by determining the minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), and by performing a time-kill assay. RESULTS: Abscisic acid related to the botanical origins of acacia honey from Korea has been analyzed using ultra-performance liquid chromatography. The MICs and MBCs of abscisic acid were 2.7± 1.3 and 6.9 ± 1.9 μg/mL, respectively. The bactericidal activity of abscisic acid (at 10.8 μg/mL corresponding to 4 × MIC) killed the organism within 36-72 h. These results suggest that abscisic acid isolated from Korean acacia honey has antibacterial activity against H. pylori. CONCLUSION: Abscisic acid isolated from Korean acacia honey can be therapeutic and may be further exploited as a potential lead candidate for the development of treatments for H. pylori-induced infections. SUMMARY: The crude acacia honey was extracted with n-hexane, dichloromethane, EtOAc, and n-butanolThe EtOAc extract yielded eight fractions and four subfractions were subsequently obtained chromatographicallyAbscisic acid was isolated from one subfractionAll the solvent extracts and fractions showed antibacterial activity against H. pyloriAbscisic acid exhibited antibacterial activity against H. pylori. Abbreviations used: MeOH: Methanol; EtOAc: Ethyl acetate; TSB: Trypticase soy broth; MIC: Minimum inhibitory concentration; MBC: Minimum bactericidal concentration; CFU: Colony-forming units; UPLC: Ultra-performance liquid chromatography; DAD: Diode array detector; UV: Ultraviolet; ODS: Octadecyl-silica; MS: Mass spectrometry; SE: Standard error. https://greenmedinfo.com/article/abscisic-acid-isolated-korean-acacia-honey-can-be-therapeutic-h-pylori-induced#comments Helicobacter Pylori Infection Honey Anti-Bacterial Agents Anti-Bacterial Agents Helicobacter Pylori Infection Honey In Vitro Study Wed, 27 Sep 2017 23:09:06 +0000 greenmedinfo 153486 at https://greenmedinfo.com Caffeic acid phenethyl ester synergistically enhances the antifungal activity of fluconazole against resistant Candida albicans. https://greenmedinfo.com/article/caffeic-acid-phenethyl-ester-synergistically-enhances-antifungal-activity-fluc n/a PMID:  Phytomedicine. 2018 Feb 1 ;40:55-58. Epub 2018 Jan 2. PMID: 29496175 Abstract Title:  Caffeic acid phenethyl ester synergistically enhances the antifungal activity of fluconazole against resistant Candida albicans. Abstract:  BACKGROUND: Owing to the increased morbidity and mortality associated with invasive fungal infections, treatments with a combination of antifungal agents are often considered. Caffeic acid phenethyl ester (CAPE), a major active component of propolis, possesses many biological activities, including antibacterial, antiviral, antioxidant, anti-inflammatory, and anticancer effects. PURPOSE: This study aimed to evaluate the interaction between CAPE and fluconazole (FLC) against Candida albicans. METHODS: Microdilution checkerboard and time-kill assays were employed to evaluate the in vitro interaction between CAPE and FLC. The data obtained from the checkerboard tests were analyzed by the fractional inhibitory concentration index (FICI).The antifungal activity of the CAPE and FLC combination was evaluated in vivo in a Caenorhabditis elegans model of infection. RESULTS: We observed that when used in combination, CAPE acted synergistically with FLC against FLC-resistant clinical isolates of C. albicans. In addition, the CAPE-FLC combination significantly extended the longevity and reduced fungal burden in C. elegans when compared with treatment with FLC or CAPE alone. CONCLUSION: These results indicate that the use of CAPE and FLC in combination has considerable therapeutic potential against resistant C. albicans. https://greenmedinfo.com/article/caffeic-acid-phenethyl-ester-synergistically-enhances-antifungal-activity-fluc#comments Caffeic acid phenethyl ester Candida Albicans Infection: Antibiotic Resistant Anti-Bacterial Agents Anti-Bacterial Agents Caffeic acid phenethyl ester Natural Substance/Drug Synergy In Vitro Study Fri, 09 Mar 2018 08:16:01 +0000 greenmedinfo 160967 at https://greenmedinfo.com Compounds from P. marsupium have antibacterial, antidiabetic, anti-inflammatory, and analgesic activities. https://greenmedinfo.com/article/compounds-p-marsupium-have-antibacterial-antidiabetic-anti-inflammatory-and-an n/a PMID:  J Intercult Ethnopharmacol. 2017 Apr-Jun;6(2):170-176. Epub 2017 Apr 12. PMID: 28512598 Abstract Title:  Phytochemical screening and study of antioxidant, antimicrobial, antidiabetic, anti-inflammatory and analgesic activities of extracts from stem wood of Pterocarpus marsupium Roxburgh. Abstract:  AIMS: The main aims of the study were to evaluate the phytochemical constituents and to study the antioxidant, antimicrobial, antidiabetic, anti-inflammatory, and analgesic activities of extracts from stem wood of Pterocarpus marsupium. MATERIALS AND METHODS: Ethanol, acetone and isopropyl alcohol (IPA) (1:1) extracts of stem wood of P. marsupium were subjected to phytochemical screening and analysis of biological activities from August 2015 to January 2016. The antioxidant assay was carried out using 2, 2-diphenyl-1-picrylhydrazyl scavenging method, antimicrobial activity testing by cup diffusion method, antidiabetic test evaluation by oral glucose tolerance test in mice, anti-inflammatory effect was evaluated by hind paw edema method in mice and analgesic test evaluation by a chemical writhing method in mice. RESULTS: The results of the study revealed that P. marsupium is a source of various phytoconstituents such as alkaloids, glycosides, saponins, tannins, proteins, carbohydrates, cardiac glycosides, flavonoids, and terpenoids. Both the acetone and IPA extract as well as the ethanol extract of stem wood of P. marsupium exhibited a dose-dependent antioxidant activity. Acetone and IPA extract showed antibacterial activity against Gram-positive bacteria, while the ethanolic extract was found to possess antidiabetic activity. The antidiabetic activity of the extract was found to be time and dose-dependent. Similarly, the acetone and IPA extract was found to have anti-inflammatory activity, which was also time and dose-dependent. Furthermore, the ethanolic extract showed analgesic activity, which was dose-dependent. The ethanolic extract was found to be nontoxic. CONCLUSIONS: Thus, this study laid sufficient background for the further research on extracts from stem wood of P. marsupium for identification, subsequent purification and isolation of compounds having antibacterial, antidiabetic, anti-inflammatory, and analgesic activities. https://greenmedinfo.com/article/compounds-p-marsupium-have-antibacterial-antidiabetic-anti-inflammatory-and-an#comments Gram-Positive Bacterial Infections Inflammation Pterocarpus marsupium Analgesics Anti-Bacterial Agents Anti-Inflammatory Agents Hypoglycemic Agents Analgesics Anti-Bacterial Agents Anti-Inflammatory Agents Dose Response Gram-Positive Bacterial Infections Hypoglycemic Agents Inflammation Plant Extracts Pterocarpus marsupium In Vitro Study Wed, 28 Jun 2017 20:52:21 +0000 greenmedinfo 149703 at https://greenmedinfo.com Honey has potential as an adjunct treatment with rifampicin for chronic wounds infected with staphylococcal biofilms. https://greenmedinfo.com/article/honey-has-potential-adjunct-treatment-rifampicin-chronic-wounds-infected-staph n/a PMID:  Front Microbiol. 2017 ;8:2653. Epub 2018 Jan 11. PMID: 29375518 Abstract Title:  Rifampicin-Manuka Honey Combinations Are Superior to Other Antibiotic-Manuka Honey Combinations in Eradicating Staphylococcus aureus Biofilms. Abstract:  Chronic wound infections are a major burden to both society and the health care industry. Bacterial biofilms are the major cause of chronic wound infections and are notoriously recalcitrant to treatments with antibiotics, making them difficult to eradicate. Thus, new approaches are required to combat biofilms in chronic wounds. One possible approach is to use drug combination therapies. Manuka honey has potent broad-spectrum antibacterial activity and has previously shown synergistic activity in combination with antibiotics against common wound pathogens, including Staphylococcus aureus. In addition, manuka honey exhibits anti-biofilm activity, thereby warranting the investigation of its potential as a combination therapy with antibiotics for the topical treatment of biofilm-related infections. Here we report the first use of MacSynergy II to investigate the response of established S. aureus (strain NCTC 8325) biofilms to treatment by combinations of Medihoney (medical grade manuka honey) and conventional antibiotics that are used for preventing or treating infections: rifampicin, oxacillin, fusidic acid, clindamycin, and gentamicin. Using checkerboard microdilution assays, viability assays and MacSynergy II analysis we show that the Medihoney-rifampicin combination was more effective than combinations using the other antibiotics against established staphylococcal biofilms. Medihoney and rifampicin were strongly synergistic in their ability to reduce both biofilm biomass and the viability of embedded S. aureus cells at a level that is likely to be significant in vivo. Other combinations of Medihoney and antibiotic produced an interesting array of effects: Medihoney-fusidic acid treatment showed minor synergistic activity, and Medihoney-clindamycin, -gentamicin, and -oxacillin combinations showed overall antagonistic effects when the honey was used at sub-inhibitory concentration, due to enhanced biofilm formation at these concentrations which could not be counteracted by the antibiotics. However, these combinations were not antagonistic when honey was used at the inhibitory concentration. Confocal scanning laser microscopy confirmed that different honey-antibiotic combination treatments could eradicate biofilms. Our results suggest that honey has potential as an adjunct treatment with rifampicin for chronic wounds infected with staphylococcal biofilms. We also show that MacSynergy II allows a comprehensive examination of the synergistic effects of honey-antibiotic combinations, and can help to identify doses for clinical use. https://greenmedinfo.com/article/honey-has-potential-adjunct-treatment-rifampicin-chronic-wounds-infected-staph#comments Manuka Honey Staphylococcus aureus infection Anti-Bacterial Agents Anti-Bacterial Agents Biofilm Manuka Honey Natural Substance/Drug Synergy Staphylococcus aureus infection In Vitro Study Wed, 31 Jan 2018 01:28:08 +0000 greenmedinfo 159029 at https://greenmedinfo.com N. sativa had in-vitro activity against S. enetrica and can be used as therapeutic. https://greenmedinfo.com/article/n-sativa-had-vitro-activity-against-s-enetrica-and-can-be-used-therapeutic n/a PMID:  Environ Toxicol Pharmacol. 2017 Dec 17 ;58:54-58. Epub 2017 Dec 17. PMID: 29289818 Abstract Title:  In vitro activity of Nigella sativa against antibiotic resistant Salmonella enterica. Abstract:  Salmonellosis is a major food-borne disease worldwide and antimicrobial resistance in Salmonella is a public health problem. Phytochemicals are alternative therapeutics to treat antibiotic resistant Salmonella. Biochemically identified Salmonella enterica of human and poultry origin (n = 10) were confirmed by polymerase chain reaction. Susceptibility to antibiotics was determined by Kirby Bauer disc diffusion method. In-vitro anti-salmonella activity of N. sativa essential oil and extracts (aqueous and methanol) was determined against antibiotic resistant isolates by well diffusion and micro broth dilution method. Cytotoxic potential of N. sativa was observed by MTT assay. In S. eneterica the highest resistance (100%) was detected against nalidixic acid and ampicillin followed by oflaxacin (80%), tetracycline, co-trimoxazole and amoxicillin (60%), ciprofloxacin (40%) and gentamicin (20%). Methanol extract of N. sativa produced zone of inhibition from 35 ± 1.00 to 17 ± 1.00 with mean MIC value ≥562.5 ± 384.1 μg/mL. Essential oil showed antibacterial activity with zone of inhibition from 20 ± 1.00 to 14 ± 1.00 mm and mean MIC value ≥1000.0 ± 322.7 μg/mL. Aqueous extract had no anti-salmonella activity. MTT results showed more than 50 percent cell survival at concentrations&gt;625 and&gt;1250 μg/mL for methanol extract and essential oil of N. sativa respectively; concentrations less than cytotoxic values required for anti-salmonella activity. It was concluded that N. sativa had in-vitro activity against S. enetrica and can be used as therapeutic. https://greenmedinfo.com/article/n-sativa-had-vitro-activity-against-s-enetrica-and-can-be-used-therapeutic#comments Bacterial Infections: Resistance/Biofilm Formation Nigella sativa (aka Black Seed) Salmonella Infections Anti-Bacterial Agents Anti-Bacterial Agents Bacterial Infections: Resistance/Biofilm Formation Nigella sativa (aka Black Seed) Salmonella Infections In Vitro Study Tue, 09 Jan 2018 19:34:20 +0000 greenmedinfo 158252 at https://greenmedinfo.com Natural isothiocyanates represent a promising group of natural anti-infective compounds with activity against Pseudomonas aeruginosa biofilms. https://greenmedinfo.com/article/natural-isothiocyanates-represent-promising-group-natural-anti-infective-compo n/a PMID:  Fitoterapia. 2017 Jun ;119:57-63. Epub 2017 Apr 5. PMID: 28390975 Abstract Title:  Natural isothiocyanates express antimicrobial activity against developing and mature biofilms of Pseudomonas aeruginosa. Abstract:  BACKGROUND: The antimicrobial properties of natural isothiocyanates (ITCs) found in plants such as nasturtium (Tropaeolum majus) and horseradish (Armoracia rusticana), and the need of new chemotherapeutic options for treatment of infections caused by multidrug-resistant and biofilm-forming Gram-negative bacteria such as Pseudomonas aeruginosa (Pa), led us to evaluate the effects of three major ITCs, allylisothiocyanate (AITC), benzylisothiocyanate (BITC), and phenylethyl-isothiocyanate (PEITC), and a mixture (ITCM) adapted to the ITC composition after release of active components out of natural sources. MATERIAL/METHODS: Out of 105Pa isolates 27 isolates with increased biofilm formation were selected for testing. The effects of ITCs on Pa were evaluated regarding (1) planktonic bacterial proliferation, (2) biofilm formation, (3) metabolic activity in mature biofilms, and (4) synergism of ITCs and antibiotics. RESULTS: (1) Each ITC had anti-Pa activity. Mean minimum inhibitory concentrations (MICs) were (μg/ml, mean±standard deviation): AITC 103±6.9; BITC, 2145±249; PEITC 29,423±1652; and ITCM, 140±5. (2) Treating bacteria with PEITC and ITCM in concentrations below the MIC significantly inhibited biofilm formation. Particularly, ITCM reduced biofilm mass and bacterial proliferation. (3) ITCssignificantly inhibited metabolic activity in mature biofilms. (4) Combining ITCs with meropenem synergistically increased antimicrobial efficacy on Pa biofilms. CONCLUSIONS: ITCs represent a promising group of natural anti-infective compounds with activity against Pa biofilms. https://greenmedinfo.com/article/natural-isothiocyanates-represent-promising-group-natural-anti-infective-compo#comments Horseradish Isothiocyanates Pseudomonas aeruginosa Anti-Bacterial Agents Anti-Bacterial Agents Biofilm Horseradish Isothiocyanates Pseudomonas aeruginosa In Vitro Study Tue, 02 Jan 2018 19:41:26 +0000 greenmedinfo 157921 at https://greenmedinfo.com Saudi honey inhibit the growth of bacterial strains and that honey can be used as complementary antimicrobial agent. https://greenmedinfo.com/article/saudi-honey-inhibit-growth-bacterial-strains-and-honey-can-be-used-complementa n/a PMID:  Vet World. 2017 Feb ;10(2):233-237. Epub 2017 Feb 21. PMID: 28344408 Abstract Title:  Potential antibacterial activity of some Saudi Arabia honey. Abstract:  AIM: The aim of this study was to investigate the potential antibacterial activity of some Saudi Arabia honey against selected bacterial strains of medical importance. MATERIALS AND METHODS: A total of 10 Saudi Arabia honey used to evaluate their antimicrobial activity against some antibiotic-resistant pathogenic bacterial strains. The bacterial strains were Staphylococcus aureus, Streptococcus pyogenes, Klebsiella pneumoniae, Escherichia coli, and Pseudomonas aeruginosa. RESULTS: The antibacterial activity of Saudi honey against five bacterial strains showed different levels of inhibition according to the type of honey. The overall results showed that the potential activity was differing according to the pathogen and honey type. CONCLUSION: It could be concluded that the Saudi honey inhibit the growth of bacterial strains and that honey can be used as complementary antimicrobial agent against selected pathogenic bacteria. https://greenmedinfo.com/article/saudi-honey-inhibit-growth-bacterial-strains-and-honey-can-be-used-complementa#comments Escherichia coli Infections Honey Klebsiella Infections Pseudomonas aeruginosa Staphylococcus aureus infection Streptococcus pyogenes Anti-Bacterial Agents Anti-Bacterial Agents Escherichia coli Infections Honey Klebsiella Infections Pseudomonas aeruginosa Staphylococcus aureus infection Streptococcus pyogenes In Vitro Study Thu, 28 Sep 2017 00:03:09 +0000 greenmedinfo 153491 at https://greenmedinfo.com The antibacterial activity of melilot honey is created by hydrogen peroxide and other bioactive compounds. https://greenmedinfo.com/article/antibacterial-activity-melilot-honey-created-hydrogen-peroxide-and-other-bioac n/a PMID:  Lett Appl Microbiol. 2017 Jul ;65(1):82-89. PMID: 28426165 Abstract Title:  Hydrogen peroxide-dependent antibacterial action of Melilotus albus honey. Abstract:  : Honey originating from different floral sources exhibits the broad spectrum of antibacterial activity as a result of the presence of hydrogen peroxide as well as nonperoxide bioactive compounds. The mechanisms of antibacterial activity of Polish melilot honey were investigated for the first time. Polish melilot honey samples (Melilotus albus biennial = 3 and annual = 5, Melilotus officinalis = 1) were collected directly from beekeepers and analysed for pollen profile, basic physicochemical parameters, antioxidant capacity, radical scavenging activity, total phenolic contents as well as antibacterial properties against pathogenic bacteriaStaphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Salmonella spp. The physicochemical properties of melilot honey were specific for light-coloured unifloral honey samples and were not dependent on its botanical and geographical origin (P &gt; 0·05). All tested honey samples exhibited inhibitory activity (above 90%) against Gram-positive bacteria at the concentration of 12·5-25%. Above 30-50% of antibacterial activity of melilot honey was connected with glucose oxidase enzyme action and was destroyed in the presence of catalase. Hydrogen peroxide-dependent antibacterial activity of honey was inversely correlated with its radical scavenging activity (r = -0·67) and phenolic compounds (r = -0·61). Antibacterial action of melilot honey depends not only on hydrogen peroxide produced by glucose oxidase, but also on other nonperoxide bioactive components of honey. SIGNIFICANCE AND IMPACT OF THE STUDY: Melilot honey is used in traditional medicine as an anticoagulant agent due to the possibility of the presence of the coumarin compounds which are specific for Melilotus plant. Melilotus albus is rarely used to produce honey, and antibacterial properties of this variety of honey had not been studied yet. Nine samples of melilot honey produced in different regions of Poland were analysed according to their antibacterial activity which was correlated with physiochemical parameters and antioxidant activity. It was shown that antibacterial activity of melilot honey is created by hydrogen peroxide and other bioactive compounds. https://greenmedinfo.com/article/antibacterial-activity-melilot-honey-created-hydrogen-peroxide-and-other-bioac#comments Escherichia coli Infections Honey Klebsiella Infections Salmonella Infections Staphylococcus aureus infection Anti-Bacterial Agents Anti-Bacterial Agents Escherichia coli Infections Honey Klebsiella Infections Salmonella Infections Staphylococcus aureus infection In Vitro Study Wed, 27 Sep 2017 23:54:14 +0000 greenmedinfo 153490 at https://greenmedinfo.com The results of this preliminary study indicate a possible role of honey eardrops in eczematous ear disease. https://greenmedinfo.com/article/results-preliminary-study-indicate-possible-role-honey-eardrops-eczematous-ear n/a PMID:  Otolaryngol Head Neck Surg. 2017 Jul 1:194599817718782. Epub 2017 Jul 1. PMID: 28695767 Abstract Title:  Treatment of Recurrent Eczematous External Otitis with Honey Eardrops: A Proof-of-Concept Study. Abstract:  Eczematous external otitis is a chronic inflammatory disease and often difficult to treat. Our objective was to investigate the clinical effect and in vitro antibacterial potential of medical honey eardrops as treatment of eczematous external otitis. In a prospective study, 15 patients diagnosed with recurrent eczematous external otitis were treated with medical honey eardrops for 2 weeks. The following clinical outcomes were evaluated: visual analog scale of ear complaints, score of eczema, and eradication of bacterial infection. Furthermore, the antibacterial effect of honey eardrops against different bacterial strains was tested in vitro. Treatment resulted in less discomfort and itching and decreased signs of eczema, with high patient satisfaction and without adverse reactions. Honey eardrops showed a strong in vitro inhibitory activity against all tested strains but did not eradicate Staphylococcus aureus infection in vivo. The results of this preliminary study indicate a possible role of honey eardrops in eczematous ear disease. https://greenmedinfo.com/article/results-preliminary-study-indicate-possible-role-honey-eardrops-eczematous-ear#comments Eczema Honey Otitis Anti-Bacterial Agents Anti-Bacterial Agents eczema Honey Otitis Human Study Human In Vitro Wed, 27 Sep 2017 23:17:42 +0000 greenmedinfo 153489 at https://greenmedinfo.com The results showed that β-caryophyllene has antimicrobial activity against the proliferation of dog's dental plaque-forming bacteria. https://greenmedinfo.com/article/results-showed-caryophyllene-has-antimicrobial-activity-against-proliferation- n/a PMID:  BMC Vet Res. 2016 Oct 1 ;12(1):216. Epub 2016 Oct 1. PMID: 27716286 Abstract Title:  Use ofβ-caryophyllene to combat bacterial dental plaque formation in dogs. Abstract:  BACKGROUND: Periodontal disease is a highly prevalent illness that affects many dogs, reaching up to 85 % prevalence in individuals over the age of 4 years. Currently the drug of choice for combating the formation of dental plaque in these animals, the etiologic agent of the disease, is chlorhexidine, which has several side effects reported. Thus, surveys are conducted throughout the world in orderto identify potential substitutes for antimicrobial therapy and prevention of periodontal disease. The objective of the work was to evaluate the antimicrobial activity of β-caryophyllene against bacteria from dog&#039;s dental plaque in vitro and in vivo. The minimum inhibitory concentration was evaluated by agar microdilution assay, the induction or inhibition of bacterial adherence by sub-inhibitory concentrations in 96-well plates, and reduction of dental plaque formation in mongrel dogs subjected to topical solution with β-caryophyllene for 15 days. RESULTS: Results showed minimum inhibitory concentrations above 100 mg/mL for 25 % of the isolates, 100 mg/mL for 3 %, 50 mg/mL for 25 %, 25 mg/mL for 12 %, 12.5 mg/mL for 19 % and 6.25 mg/mL for 16 %. Bacterial adherences of three Enterococcus sp., one Streptococcus sp., one Haemophilus sp., one Aerococcus sp., one Bacillus sp. and one Lactococcus sp.isolates were inhibited by subinhibitory concentration. One Lactococcus sp., one Bacillus sp. and one Streptococcus sp. were stimulated to adhere by concentrations of 0.19, 1.56 and 0.78 mg/mL, respectively. In vivo assay showed reduction in dental plaque formation by β-caryophyllene, with final plaque coverage of 23.3 ± 2.6 % of the total area of the teeth, with significant difference compared with chlorhexidine group (37.5 ± 3.7 % - p &lt; 0.05) and negative control group (65.5 ± 2.5 % - p &lt; 0.001). CONCLUSIONS: The results showed thatβ-caryophyllene has antimicrobial activity against the proliferation of dog&#039;s dental plaque-forming bacteria representing a suitable alternative to the use of chlorhexidine in prophylaxis and treatment of periodontal disease of dogs. https://greenmedinfo.com/article/results-showed-caryophyllene-has-antimicrobial-activity-against-proliferation-#comments Beta-Caryophyllene Dental Plaque Periodontal Diseases Anti-Bacterial Agents Anti-Bacterial Agents Caryophyllene Dental Plaque Periodontal Diseases Animal Study Tue, 16 Jan 2018 17:31:06 +0000 greenmedinfo 158462 at https://greenmedinfo.com These results suggest that dandelion extracts have potential as antibacterial uropathogenic disease agents. https://greenmedinfo.com/article/these-results-suggest-dandelion-extracts-have-potential-antibacterial-uropatho n/a PMID:  Evid Based Complement Alternat Med. 2018 ;2018:2706417. Epub 2018 Jan 1. PMID: 29507587 Abstract Title:  Isolation and Identification of Compounds from Bioactive Extracts ofWeber ex F. H. Wigg. (Dandelion) as a Potential Source of Antibacterial Agents. Abstract:  Currently, the most effective treatment for recurrent urinary tract infections in women is antibiotics. However, the limitation for this treatment is the duration and dosage of antibiotics and the resistance that bacteria develop after a long period of administration. With the aim of identifying mainly novel natural agents with antibacterial activity, the present study was undertaken to investigate the biological and phytochemical properties of extracts from the leavesThe structural identification of compounds present in hexane (Hex) and ethyl acetate (AcOEt) extracts was performed by mass spectrometry (GC-MS) spectroscopic techniques and nuclear magnetic resonance (NMR) with the major compounds corresponding to different sesquiterpene lactones (-santonin, glabellin, arborescin, and estafiatin), monoterpene (9,10-dimethyltricycle [4.2.1.1 (2,5)]decane-9,10-diol), phytosterol (Stigmasta-5,22-dien-3-ol acetate), terpenes (lupeol acetate, pregn-5-en-20-one-3-acetyloxy-17-hydroxy, 2-hydroxy-4-methoxy benzaldehyde), and coumarin (benzofuranone 5,6,7,7-a-tetraaldehyde-4,4,7a-trimethyl). The results obtained show that the Hex extract was highly active againstshowing a MIC of 200 g/mL and moderately active againstandwith MIC values of 400 g/mL and 800 g/mL for the other Gram-negative strains tested withas uropathogens. Therefore, the effective dandelion extracts could be used in the development of future products with industrial application. https://greenmedinfo.com/article/these-results-suggest-dandelion-extracts-have-potential-antibacterial-uropatho#comments Dandelion Escherichia coli Infections Klebsiella Infections Staphylococcus aureus infection Anti-Bacterial Agents Anti-Bacterial Agents Dandelion Escherichia coli Infections Klebsiella Infections Staphylococcus aureus infection Animal Study In Vitro Study Sat, 10 Mar 2018 01:57:03 +0000 greenmedinfo 160996 at https://greenmedinfo.com This review discusses both the current knowledge of and new perspectives for honey therapy in ophthalmology. https://greenmedinfo.com/article/review-discusses-both-current-knowledge-and-new-perspectives-honey-therapy-oph n/a PMID:  Forsch Komplementmed. 2016 ;23(6):364-369. Epub 2016 Nov 3. PMID: 27924791 Abstract Title:  Honey: A Natural Remedy for Eye Diseases. Abstract:  Honey has been considered as a therapeutic agent; its successful application in the treatment of non-healing infected wounds has promoted its further clinical usage for treating various disorders including eye disorders. There is evidence that honey may be helpful in treating dry eye disease, post-operative corneal edema, and bullous keratopathy. Furthermore, it can be used as an antibacterial agent to reduce the ocular flora. This review discusses both the current knowledge of and new perspectives for honey therapy in ophthalmology. https://greenmedinfo.com/article/review-discusses-both-current-knowledge-and-new-perspectives-honey-therapy-oph#comments Eye Diseases Honey Wound Healing Anti-Bacterial Agents Anti-Inflammatory Agents Anti-Bacterial Agents Anti-Inflammatory Agents Eye Diseases Honey wound healing Review Thu, 28 Sep 2017 00:21:27 +0000 greenmedinfo 153492 at https://greenmedinfo.com Thymoquinone may be considered for topical application alone in the treatment of acute otitis externa. https://greenmedinfo.com/article/thymoquinone-may-be-considered-topical-application-alone-treatment-acute-otiti n/a PMID:  J Int Adv Otol. 2017 Dec 14. Epub 2017 Dec 14. PMID: 29283096 Abstract Title:  The Role of Topical Thymoquinone in the Treatment of Acute Otitis Externa; an Experimental Study in Rats. Abstract:  OBJECTIVE: The aim of this experimental study was to compare the dose-related effect of topical thymoquinone (TQ) with other topical agents used in the management of acute otitis externa (AOE) in a rat model. MATERIALS AND METHODS: Forty-eight male Wistar albino rats were divided into six groups each with eight rats per group. Group I was the control group with no external otitis, whereas external otitis were created in the other five groups (study groups). Dexamethasone, 0.1% TQ, 0.4% TQ, ciprofloxacin, and 0.9% saline (NaCl) drops was applied once daily in Groups II-VI, respectively. The treatment was administered regularly for 10 days. Pathologic and microbiologic evaluation were performed. Pathologically, the thicknesses of the stroma and the epithelium in the external auditory canal (EAC) were measured using an occulometer. Edema in the stroma, density of inflammatory cells and blood vessels, presence of fibroblasts, and changes in collagen fibers in the EAC were evaluated in five different areas to obtain the area of highest concentration and classified into four grades (0=no change, 1=mild, 2=moderate, 3=severe). RESULTS: The higher concentration of TQ (0.4%) was more effective than dexamethasone and 0.1% TQ with respect to antibacterial and the anti-inflammatory properties. CONCLUSION: TQ, particularly at a concentration of 0.4%, may be considered for topical application alone in the treatment of AOE, without any requirement for a combined treatment. https://greenmedinfo.com/article/thymoquinone-may-be-considered-topical-application-alone-treatment-acute-otiti#comments Otitis Thymoquinone Anti-Bacterial Agents Anti-Inflammatory Agents Anti-Bacterial Agents Anti-Inflammatory Agents Otitis Superiority of Natural Substances versus Drugs thymoquinone Animal Study Tue, 09 Jan 2018 19:53:27 +0000 greenmedinfo 158254 at https://greenmedinfo.com Tomatidine, a lead antibiotic molecule that targets Staphylococcus aureus ATP synthase subunit C. https://greenmedinfo.com/article/tomatidine-lead-antibiotic-molecule-targets-staphylococcus-aureus-atp-synthase n/a PMID:  Antimicrob Agents Chemother. 2018 Apr 2. Epub 2018 Apr 2. PMID: 29610201 Abstract Title:  Tomatidine, a lead antibiotic molecule that targetsATP synthase subunit C. Abstract:  Methicillin-resistant(MRSA) is a leading cause of deadly hospital-acquired infections. The discovery of anti-antibiotics and new classes of drugs not susceptible to the mechanisms of resistance shared among bacteria is imperative. We recently showed that tomatidine (TO), a steroidal alkaloid from solanaceous plants, possesses potent antibacterial activity againstsmall colony variants (SCVs), the notoriously persistent form of this bacterium that has been associated with recurrence of infections. Here, using genomic analysis of-generated TO-resistantstrains to identify mutations in genes involved in resistance, we identified the bacterial ATP synthase as the cellular target. Sequence alignments were performed to highlight the modified sequences and the structural consequences of the mutations were evaluated in structural models. Overexpression of thegene inSCVs or introducing the mutation found in thegene of one of the high-level TO-resistantmutant into thegene provided resistance to TO and further validated the identity of the cellular target. FC04-100, a TO derivative which also possesses activity against non-SCV strains, prevents high-level resistance development in prototypic strains and limits the level of resistance observed in SCVs. An ATP synthesis assay allowed the observation of a correlation between antibiotic potency and ATP synthase inhibition. The selectivity index (inhibition of ATP production by mitochondriabacterial ATP synthase) is estimated to be&gt;10-fold for FC04-100. https://greenmedinfo.com/article/tomatidine-lead-antibiotic-molecule-targets-staphylococcus-aureus-atp-synthase#comments Staphylococcus aureus: Methicillin-resistant (MRSA) Tomatidine Anti-Bacterial Agents Anti-Bacterial Agents Staphylococcus aureus: Methicillin-resistant (MRSA) Tomatidine In Vitro Study Sat, 07 Apr 2018 02:26:49 +0000 greenmedinfo 162317 at https://greenmedinfo.com Toxic Detergents vs Soap Nuts A Laundry Soap You Can Eat https://greenmedinfo.com/blog/toxic-detergents-vs-soap-nuts-laundry-soap-you-can-eat <div class="copyright">This article is copyrighted by GreenMedInfo LLC, 2013<br/><strong><a href="/greenmedinfocom-re-post-guidelines">Visit our Re-post guidelines</a></strong></div><p class="rtecenter" dir="ltr"><img alt="Toxic Detergents vs Soap Nuts A Laundry Soap You Can Eat" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/ken@sinfullywholesome.com/images/whats%20in%20your%20laundry%20soap%20baby%20skull.jpg" /></p> <p><span style="font-size:14px;"><span style="font-family:arial,helvetica,sans-serif;">How we do our laundry, clean our homes and bathe ourselves has changed more in the last 70 years than it has in the previous 7,000 years. None of these changes have been kind to your skin, or good for your health.</span></span></p> <p><span style="font-size:14px;"><span style="font-family:arial,helvetica,sans-serif;">The name brand chemical based laundry detergents, household cleaners, soaps, and shampoos &nbsp;that you use in your day to day life contain carcinogens, neurotoxins and envirotoxins.</span></span></p><p><a href="https://greenmedinfo.com/blog/toxic-detergents-vs-soap-nuts-laundry-soap-you-can-eat" target="_blank">read more</a></p> https://greenmedinfo.com/blog/toxic-detergents-vs-soap-nuts-laundry-soap-you-can-eat#comments Cancer Candida Albicans Candida Infection Candidiasis Candidiasis: Vulvovaginal Carcinoma: Non-Small-Cell Lung Carcinoma: Small Cell Lung Chemical Poisoning Chemical Poisoning: Accumulated Environmental Toxins Childhood Chronic Lung Diseases Dandruff Diaper Rash Eczema Eczema: Atopic Eczema: Childhood Eczema: Infant Head Lice Inflammations Melanoma Parapsoriasis Rashes Trichomoniasis 1 4 - Dioxane acetaldehyde Allergenic anti aging anti microbial Anti-Anxiety Agents Anti-Bacterial Agents Anti-cancer Anti-Fertility anti-fungal Anti-fungal effects Anti-Trichomonas Antioxidant Antioxidant Effects Anxiolytic activity Benzene Cancer Carcinogen Carcinogenic Carcinogenic (suspected) Chemical and Drug Toxicity Corrosive Dehydrating Endocrine disruption: Leptin resistance Endocrine Disruptor Endocrine Disruptor: Insulin Resistance Endocrine Disruptor: Ovary Endocrine Disruptor: Pancreas Endocrine Disruptor: Pituitary Endocrine Disruptor: Testes Endocrine Disruptor: Thyroid Endocrine Disruptor: Uterus For Goodness Sake Gastrotoxic Health Guide: Aging Health Guide: Atmospheric Aerosols/Geoengineering Health Guide: Breast Cancer Health Guide: Chemical Exposures Health Guide: Herbs and Traditional Knowledge Health Guide: Medicinal Spices Health Guide: Mental Health Health Guide: Parenting Health Guides: Healing Foods Hepatoprotective Infant and Children's Heatlh Inflammatory Insecticidal Irritant Men's Health Molluscicidal Nonylphenol Ethoxylate (NPE) Oxidant Phosphates Skin Allegy Testing Sodium Lauryl Sulfate (SLS) Spermicidal Activity Tyrosinase Inhibition VOC Volatile organic compounds Women's Health anti aging anti microbial Anti-Anxiety Agents Anti-bacterial Anti-Bacterial Agents Anti-cancer anti-fungal Anti-fungal effects Anti-Trichomonas Antioxidant Antioxidant effects Anxiolytic activity Cancer Candida Albicans Candida Infection Candidiasis Candidiasis: Vulvovaginal Carcinoma: Non-Small-Cell Lung Carcinoma: Small Cell Lung Chemical Poisoning Chemical Poisoning: Accumulated Environmental Toxins Childhood Chronic Lung Diseases Dandruff Diaper Rash dryer sheets eczema Eczema: Atopic Eczema: Childhood Eczema: Infant fabric softeners Head Lice Hepatoprotective Inflammations Insecticidal laundry detergent laundry soap melanoma Molluscicidal Parapsoriasis Rashes Sapindus Sapindus mukorossi soap berry soap nut soap nuts soapberry soapnut soapnuts Spermicidal activity Trichomoniasis Tyrosinase inhibition Tue, 29 Oct 2013 19:41:36 +0000 sinfullywholesome 110143 at https://greenmedinfo.com