n/a
Article Publish Status: FREE
Abstract Title:

Thymoquinone Induced Leishmanicidal Effect via Programmed Cell Death in.

Abstract Source:

ACS Omega. 2022 Mar 29 ;7(12):10718-10728. Epub 2022 Mar 15. PMID: 35382308

Abstract Author(s):

Mohammad Islamuddin, Abuzer Ali, Obaid Afzal, Amena Ali, Intzar Ali, Abdulmalik Saleh Alfawaz Altamimi, Mubarak A Alamri, Kentaro Kato, Shama Parveen

Article Affiliation:

Mohammad Islamuddin

Abstract:

Visceral leishmaniasis (VL) or kala-azar is a vector-borne dreaded protozoal infection that is caused by the parasite. With increases in the dramatic infection rates, present drug toxicity, resistance, and the absence of an approved vaccine, the development of new antileishmanial compounds from plant sources remains the keystone for the control of visceral leishmaniasis. In this study, we evaluated the leishmanicidal effect of thymoquinone againstwith anandmodel. Thymoquinone exhibited potent antipromastigote activity with ICand ICconcentrations achieved at 6.33± 1.21 and 20.71 ± 2.15 μM, respectively, whereas the ICand ICconcentrations were found to be 7.83± 1.65 and 27.25 ± 2.20 μM against the intramacrophagic form of amastigotes, respectively. Morphological changes in promastigotes and growth reversibility study following treatment confirmed the leishmanicidal effect of thymoquinone. Further, thymoquinone exhibited leishmanicidal activities againstpromastigote through cytoplasmic shrinkage, membrane blebbing, chromatin condensation, cellular and nuclear shrinkage, and DNA fragmentation, as observed under scanning and transmission electron microscopy analyses. The antileishmanial activity was exerted via programmed cell death as proved by exposure of phosphatidylserine, DNA nicking by TUNEL assay, and loss of mitochondrial membrane potential. Thymoquinone at a concentration of 200μM was devoid of any cytotoxic effects against mammalian macrophage cells. Thymoquinone showed strong leishmanicidal activity against, which is mediated via an apoptosis mode of parasitic cell death, and accordingly, thymoquinone may be the source of a new lead molecule for the cure of VL.

Study Type : In Vitro Study
Additional Links
Pharmacological Actions : Leishmanicidal : CK(118) : AC(106)

Print Options


Key Research Topics

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2024 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.