Article Publish Status: FREE
Abstract Title:

Theaflavin promoted apoptosis in nasopharyngeal carcinoma unexpectedly via inducing autophagy.

Abstract Source:

Iran J Basic Med Sci. 2022 Jan ;25(1):68-74. PMID: 35656444

Abstract Author(s):

Jing Xu, Shu-Juan Wang, Shan-Shan Bu, Xiao-Qi Guo, Hong Ge

Article Affiliation:

Jing Xu


Objectives: This study aimed to investigate the mechanism of the anticancer effect of theaflavin (TF) in nasopharyngeal carcinoma.

Materials and Methods: CNE2 cells were used to study the anticancer effect of TF. This study used Cell Counting Kit-8 (CCK8) assay on proliferation and used flow cytometry to detect apoptosis. The protein expression of Bcl-2, Bax, caspase 3, and caspase 9 was detected by Western blot, and autophagy-related proteins were also detected.

Results: TF inhibited proliferation of CNE2 cells, promoted apoptosis, and up-regulated the expression of caspase 3, caspase 9, and Bax, and decreased the level of Bcl-2. Unexpectedly, TF induced autophagy rather than inhibiting autophagy through up-regulating the levels of the autophagy marker light chain 3 (LC3) and Lysosomal-associated membrane protein 1 (LAMP1) and reducing levels of the autophagosome cargo protein p62, and the effect was via the mTOR pathway. Besides, autophagy inhibitor Chloroquine (CQ) suppressed the effect of TF on Bax, Bcl-2 and activation of caspase 3 and caspase 9.

Conclusion: TF promoted apoptosis of nasopharyngeal carcinoma cells, the mechanism was unexpectedly involved in inducing autophagy.

Study Type : In Vitro Study

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Sayer Ji
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