Abstract Title:

[Determination of eleutheroside B in antifatigue fraction of Acanthopanax senticosus by HPLC].

Abstract Source:

Zhongguo Zhong Yao Za Zhi. 2008 Dec;33(23):2800-2. PMID: 19260316

Abstract Author(s):

Chen Li, Xiao-yan Wang, Xu-wei Hu, Hai-tao Fang, Shan-yi Qiao

Article Affiliation:

Shenyang Pharmaceutical University, shenyang 110016, China.

Abstract:

OBJECTIVE: To develop an HPLC method for the determination of eleutheroside B in As1 (the extract fraction from Acanthopanax senticosus, which has a good effect of antifatigue). METHOD: The antifatigue effect of Asl was evaluated by mice burden swimming testing. One compound was isolated by silica gel, Sephadex LH-20 column chromatography and preparative high performance liquid chromatography. The structure was identified by physicochemical properties and spectral evidences. Eleutheroside B in Asl was determined by HPLC. Chromatographic conditions included Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) and the mobile phase consisting of a mixture of acetonitrile-water-ethanoic acid (10: 90: 0.01). The UV detection wavelength was set at 344 nm. RESULT: As1 showed an excellent antifatigue activity; One compound was isolated from As1 and its structure was identified as Eleutheroside B; The calibration cure was linear in the range of 0.104-20.8 microg (r = 0.9999), the average recovery was 97.68%, RSD 1.4% (n=6). CONCLUSION: This HPLC method is simple,accurate and reliable.

Study Type : Animal Study

Print Options


Key Research Topics

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2024 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.