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Article Publish Status: FREE
Abstract Title:

Antioxidant Extracts of Nettle () Leaves: Evaluation of Extraction Techniques and Solvents.

Abstract Source:

Molecules. 2022 Sep 15 ;27(18). Epub 2022 Sep 15. PMID: 36144748

Abstract Author(s):

María Flórez, Patricia Cazón, Manuel Vázquez

Article Affiliation:

María Flórez

Abstract:

Nettle () is a great source of bioactive compounds. The objective of this study was to evaluate the extraction techniques (ultrasound, without stirring, and stirring), solvents (methanol, water, and ethanol), and extraction times (1-4 h) to maximize antioxidant capacity of theextracts. In the case of total phenolic content (TPC) and ABTS(2,2-azino-bis(3-etilbenzotiazolin)-6-sulfonic acid) free radical scavenging values, ultrasound extraction was the most efficient method, while the best results of DPPH(1,1-diphenyl-2-picrylhydrazyl) assay in nettle extracts (91.08%) were obtained using stirring extraction, water as solvent, and 3 h of extraction time. Based on the obtained mathematical models, the optimization revealed that the best extraction conditions were ultrasound treatment with water as solvent and an extraction time of 3.15 h, obtaining values of 21.9 mg eq gallic acid/g dried nettle for TPC, 71.8% for %ABTSand 86.6% for %DPPH. This work proves that aqueous extract of nettle leaves through the ultrasound technique is an important source of natural antioxidants and can be considered a potential alternative to synthetic antioxidants.

Study Type : In Vitro Study

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