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Abstract Title:

In Vitro Growth- and Encystation-Inhibitory Efficacies of Matcha Green Tea and Epigallocatechin Gallate Against.

Abstract Source:

Pathogens. 2020 Sep 17 ;9(9). Epub 2020 Sep 17. PMID: 32957663

Abstract Author(s):

Ameliya Dickson, Elise Cooper, Lenu B Fakae, Bo Wang, Ka Lung Andrew Chan, Hany M Elsheikha

Article Affiliation:

Ameliya Dickson

Abstract:

We examined the inhibitory effect of matcha green tea () and epigallocatechin gallate (EGCg; the most abundant catechin in tea) on the vegetative growth and encystation ofT4 genotype. The sulforhodamine B (SRB) stain-based colorimetric assay and hemocytometer counting were used to determine the reduction introphozoite proliferation and encystation, in response to treatment withor EGCg. Fourier transform infrared (FTIR) microscopy was used to analyze chemical changes in the trophozoites and cysts due totreatment. Hot brewed and cold brewed matcha inhibited the growth of trophozoites by>40% at a 100 % concentration. EGCg at concentrations of 50 to 500µM significantly inhibited the trophozoite growth compared to control. Hot brewed matcha (100% concentration) also showed an 87% reduction in the rate of encystation compared to untreated control. Although 500 µM of EGCg increased the rate of encystation by 36.3%, 1000 µM reduced it by 27.7%. Both percentages were not significant compared to control.induced more cytotoxicity to Madin Darby canine kidney cells compared to EGCg. FTIR chemical fingerprinting analysis showed that treatment with brewed matcha significantly increased the levels of glycogen and carbohydrate in trophozoites and cysts.

Study Type : In Vitro Study

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