Isolation and identification of antimicrobial substances from Korean Lettuce. - GreenMedInfo Summary
Isolation and identification of antimicrobial substances from Korean Lettuce (M.).
Food Sci Biotechnol. 2018 Jun ;27(3):789-797. Epub 2018 Jan 16. PMID: 30263805
Tae Yean Hwang
The aim of this study was to identify of antimicrobial substances from Korean Lettuce (M.). Water and ethanol extracts ofM. exhibited antimicrobial activities against the microorganisms tested. Ethanol extract showed strong antimicrobial activities against most Gram positive and Gram negative microorganisms, whereas no lactic acid bacteria and yeasts were not affected. The antimicrobial compound G-6 was isolated from the ethyl acetate fraction obtained by silica gel column chromatography and HPLC and was confirmed as stable against heat treatment. Molecular weight of G-6 was calculated as 154 kDa based on information in the MS spectrum. G-6 was identified as 2-nonynoic acid (CHO) by UV, LC-EI/MS, and LC-CI/MS. In support of this, the chromatogram of G-6 was consistent with that of the 2-nonynoic acid standard. Antimicrobial activity of 2-nonynoic acid was identified in all samples, with a significant difference based on concentratio.