Inhibitory effects of Inonotus obliquus polysaccharide on inflammatory response in Toxoplasma gondii. - GreenMedInfo Summary
Inhibitory Effects ofPolysaccharide on Inflammatory Response inInfected RAW264.7 Macrophages.
Evid Based Complement Alternat Med. 2021 ;2021:2245496. Epub 2021 Dec 29. PMID: 35003292
Kexin Yan
Our previous reports have shown thatpolysaccharide (IOP) has protective effects against() infection. The aim of the present research is to explore theanti-inflammatory effects of IOP and its mechanism in RAW264.7 macrophages infected by. In this study, it is indicated that IOP decreased the excessive secretion of inflammatory cytokines tumor necrosis factor-(TNF-), interferon-(IFN-), interleukin-1(IL-1), IL-4, and IL-6 in-infected RAW264.7 macrophages. IOP effectively suppressed the mRNA expression of these cytokines and chemokines monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1(MIP-1). Moreover, IOP inhibited the phosphorylation of inhibitor kappa B kinase/(IKK/), inhibitorB(IB), p65 in nuclear factor-kappa B (NF-B) signaling pathway and p38, c-Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase 1/2 (ERK1/2) in mitogen-activated protein kinases (MAPKs) signaling pathway. Meantime, IOP prevented NF-B p65 and c-Jun translocation from the cytoplasm to the nucleus. Further, IOP downregulated the protein expression of toll-like receptor 2 (TLR2) and TLR4 in-infected RAW264.7 macrophages. The above results suggest that IOP can inhibit the inflammatory response infected withvia regulating TLR2/TLR4-NF-B/MAPKs pathways and exerting its anti-role.