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Abstract Title:

Infrared neural stimulation induces intracellular Carelease mediated by phospholipase C.

Abstract Source:

J Biophotonics. 2018 Feb ;11(2). Epub 2017 Aug 9. PMID: 28700117

Abstract Author(s):

David Moreau, Claire Lefort, Jolien Pas, Sylvia M Bardet, Philippe Leveque, Rodney P O'Connor

Article Affiliation:

David Moreau

Abstract:

The influence of infrared laser pulses on intracellular Casignaling was investigated in neural cell lines with fluorescent live cell imaging. The probe Fluo-4 was used to measure Cain HT22 mouse hippocampal neurons and nonelectrically excitable U87 human glioblastoma cells exposed to 50 to 500 ms infrared pulses at 1470 nm. Fluorescence recordings of Fluo-4 demonstrated that infrared stimulation induced an instantaneous intracellular Catransient with similar dose-response characteristics in hippocampal neurons and glioblastoma cells (half-maximal effective energy density ECof around 58 J.cm). For both type of cells, the source of the infrared-induced Catransients was found to originate from intracellular stores and to be mediated by phospholipase C and IP-induced Carelease from the endoplasmic reticulum. The activation of phosphoinositide signaling by IR light is a new mechanism of interaction relevant to infrared neural stimulation that will also be widely applicable to nonexcitable cell types. The prospect of infrared optostimulation of the PLC/IPcell signaling cascade has many potential applications including the development of optoceutical therapeutics.

Study Type : In Vitro Study

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