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Article Publish Status: FREE
Abstract Title:

Icariin alleviates osteoarthritis through PI3K/Akt/mTOR/ULK1 signaling pathway.

Abstract Source:

Eur J Med Res. 2022 Oct 17 ;27(1):204. Epub 2022 Oct 17. PMID: 36253872

Abstract Author(s):

Yan Chen, Xiaoli Pan, Jing Zhao, Chunyan Li, Yupei Lin, Yu Wang, Xu Liu, Mei Tian

Article Affiliation:

Yan Chen

Abstract:

OBJECTIVES: This study aims to investigate the effects of Icariin (ICA) on interleukin-1β(IL-1β)-induced osteoarthritis (OA) and its potential mechanism of action.

METHODS: SW1353 chondrocytes were pretreated with ICA for 2 h, followed by stimulation with IL-1βto mimic OA. Expression levels of matrix metalloproteinases (MMP-3) and collagen II were determined using real-time PCR and Western blot assays. Autophagy activation (by ICA) or inhibition (by shRNA) was determined based on the expression levels of ULK1, Beclin-1, LC3-II/I, and p62, using Western blot analysis. The phosphorylation levels of PI3K, Akt, mTOR, and ULK1 were also detected using Western blot analysis.

RESULTS: IL-1βincreased MMP-3 overproduction, induced collagen II degradation, and reduced the level of autophagy-associated proteins, including ULK1, Beclin-1, and LC3-II/I. In contrast, ICA pretreatment attenuated IL-1β-induced MMP-3 overproduction, increased collagen II expression, and induced expression of autophagy-related proteins. ICA also decreased PI3K, Akt, and mTOR phosphorylation, increased the production of ULK1, and induced autophagy. Short hairpin RNA-mediated knockdown of ULK1 led to activation of the PI3K/Akt/mTOR pathway, which reversed the protective effects of ICA.

CONCLUSIONS: Our findings indicate that ICA can induce autophagy by regulating the PI3K/AKT/mTOR/ULK1 signaling pathway. This study suggests that ICA may be effective for treating OA.

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