Effects of two polysaccharides from Lepidium meyenii (maca) on intestinal immunity and inflammation in vitro. - GreenMedInfo Summary
Effects of two polysaccharides from(maca) on intestinal immunity and inflammation.
Food Funct. 2022 Mar 21 ;13(6):3441-3452. Epub 2022 Mar 21. PMID: 35244109
Wenbo Chen
In our previous studies, two polysaccharides (MC-1 and MC-2) were identified in the roots of maca (). In this study, the effects of these two polysaccharides on intestinal immunity and inflammation were assessed using intestinal epithelial cells,, Caco-2 cells. The levels of cytokines and tight junction proteins were measured using ELISA and qPCR, respectively. The results showed that MC-1 and MC-2 were difficult to be absorbed in the Caco-2 cell monolayer, but promoted the secretion of IL-8, IL-10, IL-12, and INF-γ in the Caco-2 cells, and the secretion of TNF-α, IL-6, and NO in macrophages (RAW264.7 cells)the Caco-2 cell monolayer. Additionally, both polysaccharides inhibited damage induced by lipopolysaccharides to the densification of the Caco-2 monolayer membrane by regulating the expressions of TLR4, ZO-1, and occludin. Furthermore, the secretion of inflammatory factors such as TNF-α, IL-8, and INF-γ was inhibited, and the secretion of the anti-inflammatory factor, IL-10, increased. The results indicated that MC-1 and MC-2 can activate intestinal immunity and alleviate intestinal inflammation.