n/a
Article Publish Status: FREE
Abstract Title:

Effects of naringin on reversing cisplatin resistance and the Wnt/-catenin pathway in human ovarian cancer SKOV3/CDDP cells.

Abstract Source:

J Int Med Res. 2020 Oct ;48(10):300060519887869. PMID: 33086930

Abstract Author(s):

Hong Zhu, Xia Zou, ShiXin Lin, Xin Hu, Jun Gao

Article Affiliation:

Hong Zhu

Abstract:

OBJECTIVE: Ovarian cancer is one of three malignant tumors of the female reproductive system. Our previous studies showed that the traditional Chinese medicine naringin significantly inhibited the proliferation of platinum-resistant ovarian cancer cells, and that the mechanism may be related to the NF-κB pathway.

METHODS: The MTT assay was used to detect the sensitivity of SKOV3 and SKOV3/CDDP cells to cisplatin, the effect of different naringin concentrations on the proliferation of SKOV3/CDDP cells, and the reversal of cisplatin resistance in naringin-treated SKOV3/CDDP cells. Western blotting was used to detectβ-catenin, c-Myc, and cyclin D1 protein levels in the different cell lines.

RESULTS: MTT results showed that different concentrations of naringin inhibited the proliferation of SKOV3 and SKOV3/CDDP cells, and that the inhibition increased with increasing concentrations and prolonged incubation times. Western blotting revealed that compared with controls (SKOV3/CDDP-0),β-catenin, c-Myc and cyclin D1 proteins levels were significantly decreased in SKOV3/CDDP-C, SKOV3/CDDP-N 20, and SKOV3/CDDP-CN 20 cells, suggesting that naringin inhibited the proliferation of SKOV3/CDDP cells in a concentration and time dependent manner.

CONCLUSIONS: Non-cytotoxic naringin reduced the expression ofβ-catenin, c-Myc, and cyclin D1 in SKOV3/CDDP cells and partially reversed cisplatin resistance in SKOV3/CDDP CN 20 cells.

Study Type : In Vitro Study

Print Options


Key Research Topics

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2024 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.