Abstract Title:

Damaging effects of gliadin on three-dimensional cell culture model.

Abstract Source:

World J Gastroenterol. 2005 Oct 14 ;11(38):5973-7. PMID: 16273608

Abstract Author(s):

Ersilia Dolfini, Luca Elli, Leda Roncoroni, Barbara Costa, Maria-Pia Colleoni, Vito Lorusso, Simona Ramponi, Paola Braidotti, Stefano Ferrero, Maria-Letizia Falini, Maria-Teresa Bardella

Article Affiliation:

Department of Gastroenterology, Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena IRCCS, Via F. Sforza 35, Milan 20122, Italy.

Abstract:

AIM: To evaluate the effects of gliadin on the oxidative environment in the"in vivo-like"model of a three-dimensional cell culture system.

METHODS: LoVo cell line (intestinal adenocarcinoma) multicellular spheroids were treated with digested gliadin (with albumin used as a control). Spheroid volumes, cell viability and morphology, lactate dehydrogenase (LDH) release, content of reduced glutathione (GSH) and activity of GSH-related enzymes were examined. The data were statistically analyzed using the Student's t-test. was considered statistically significant.

RESULTS: Gliadin reduced cell viability (from 20% to 60%) and led to morphological alterations characterized by apoptotic findings and cytoskeletal injuries. LDH activity increased. The content of GSH reduced (-20% vs controls), and activity of GSH-related enzymes was significantly inhibited.

CONCLUSION: Gliadin treatment induces an imbalance in the antioxidative mechanism of cells cultured by the three-dimensional technique. This alteration may explain the cell damage directly caused by gliadin and the subsequent morphological abnormalities.

Study Type : In Vitro Study

Print Options


Key Research Topics

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2024 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.