Cardioprotective effect of Ulmus wallichiana in β-adrenergic agonist induced cardiac hypertrophy. - GreenMedInfo Summary

Planchon (Family: Ulmaceae), a traditional medicinal plant, was used in fracture healing in the folk tradition of Uttarakhand, Himalaya, India. The present study investigated the cardioprotective effect of ethanolic extract (EE) and butanolic fraction (BF) ofin isoprenaline (ISO) induced cardiac hypertrophy in Wistar rats. Cardiac hypertrophy was induced by ISO (5 mg/kg/day, subcutaneously) in rats. Treatment was performed by oral administration of EE and BF of(500 and 50 mg/kg/day). The blood pressure (BP) and heart rate (HR) were measured by non-invasive blood pressure measurement technique. Plasma renin, Ang II, NO, and cGMP level were estimated using an ELISA kit. Angiotensin converting enzyme activity was estimated. BP and HR were significantly increased in ISO group (130.33± 1.67 mmHg vs. 111.78 ± 1.62 mmHg,<0.001 and 450.51± 4.90 beats/min vs. 347.82 ± 6.91 beats/min, respectively,<0.001). The BP and HR were significantly reduced (EE: 117.53± 2.27 mmHg vs. 130.33 ± 1.67 mmHg,<0.001, BF: 119.74± 3.32 mmHg vs. 130.33 ± 1.67 mmHg,<0.001); HR: (EE: 390.22± 8.24 beats/min vs. 450.51 ± 4.90 beats/min,<0.001, BF: 345.38± 6.79 beats/min vs. 450.51 ± 4.90 beats/min,<0.001) after the treatment of EE and BF of, respectively. Plasma renin, Ang II, ACE activity was decreased and NO, cGMP level were increased. The EE and BF ofdown regulated the expression of ANP, BNP, TNF-α, IL-6, MMP9, β1-AR, TGFβ1 and up regulated NOS3, ACE2 and Mas expression level, respectively. Thus, this study demonstrated thathas cardioprotective effect against ISO induced cardiac hypertrophy.