Abstract Title:

Vitamin C promotes pluripotency of human induced pluripotent stem cells via the histone demethylase JARID1A.

Abstract Source:

Biol Chem. 2016 Jun 25. Epub 2016 Jun 25. PMID: 27343473

Abstract Author(s):

Wassim Eid, Wafaa Abdel-Rehim

Article Affiliation:

Wassim Eid

Abstract:

Somatic cells can be reprogrammed into induced pluripotent stem (iPS) cells by defined factors, which provide a powerful basis for personalized stem-cell based therapies. However, cellular reprogramming is an inefficient and metabolically demanding process commonly associated with obstacles that hamper further use of this technology. Spontaneous differentiation of iPS cells cultures represents a significant hurdle that hinder obtaining high quality iPS cells for further downstream experimentation. In this study, we found that a natural compound, vitamin C, augmented pluripotency in iPS cells and reduced unwanted spontaneous differentiation during iPS cells maintenance. Gene expression analysis showed that Vitamin C increased the expression of the histone demethylase JARID1A. Furthermore, through gain- and loss-of-function approaches, we show that JARID1A is a key effector in promoting pluripotency and reducing differentiation downstream of vitamin C. Our results therefore highlight a straightforward method for improving the pluripotency and quality of iPS cells; it also shows a possible role for H3K4me2/3 in cell fate determination and establishes a link between vitamin C and epigenetic regulation.

Study Type : In Vitro Study

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