Urolithin A showed a strong potential of anti-aging. - GreenMedInfo Summary

Although the health benefits attributed to urolithin A, such as anti-cancer, anti-inflammatory, and antioxidant effects, are based on numerous, diverse studies carried out in vitro, the biological effects of urolith A are still not entirely understood. In this study, we explored the biological effects of urolithin A using senescent human skin fibroblasts to determine whether urolithin A has any anti-aging potential. Our results showed that urolithin A significantly increased type I collagen expression, and reduced matrix metalloproteinase-1(MMP-1) expression. Urolithin A also reduced intracellular reactive oxygen species (ROS), which may be partially due to activation of the Nrf2-mediated antioxidative response. These results indicate that urolithin A is a promising anti-aging agent. Meanwhile, we noticed that the 50μM urolithin A could cause changes in cell morphology and inhibition in cell proliferation which was due to cell-cycle arrest in G2/M phase. However, SA-β-gal staining and γH2AX immunofluorescence staining showed cellular senescence status of HSFs did not change. Results of DAPI staining (no significant change), increased Bcl-2 gene expression and mitochondrial membrane potential (no significant change) after urolithin A treatment showed that the cells did not undergo apoptosis. These results provided further insights into the molecular mechanism of urolithin A. In conclusion, urolithin Ashowed a strong potential of anti-aging.