Abstract Title:

[Protective effect ofagainst oxidative stress in neonatal mice with necrotizing enterocolitis].

Abstract Source:

Nan Fang Yi Ke Da Xue Xue Bao. 2019 Oct 30 ;39(10):1221-1226. PMID: 31801706

Abstract Author(s):

Jia Tang, Chunbao Guo, Fang Gong

Article Affiliation:

Jia Tang


OBJECTIVE: To investigate the protective effect ofDSM17938 strain against oxidative stress in a neonatal mouse model of necrotizing enterocolitis (NEC) and explore the possible mechanism.

METHODS: Ninety-six 10-day-old neonatal C57BL/6J mice were equally randomized into control group, NEC group, and NEC+group. The pathological changes of the ileocecal intestinal tissue were evaluated with HE staining and double-blind pathological scoring. The mRNA and protein expressions of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the intestinal tissues were detected using quantitative real-time PCR and ELISA, respectively. Colorimetric assays were used to determine the activity of superoxide dismutase (SOD) and its inhibition rate, malondialdehyde (MDA), glutathione (GSH), oxidizedglutathione (GSSG), and GSSG/ GSH ratio.

RESULTS: Compared with those in the control group, the neonatal mice in NEC group showed significant weight loss (<0.05), obvious intestinal injury, increased pathological scores (<0.05), increased expressions of TNF-α and IL-1β mRNA and proteins (<0.05), decreased SOD activity and inhibition rate, decreased GSH, and significantly increased MDA, GSSG, and GSSG/GSH ratios (<0.05). Treatment withobviously decreased the pathological scores, expressions of TNF-α and IL-1β (<0.05), MDA, GSSG, and GSSG/GSH ratio (<0.05), and significantly increased SOD activity, its inhibition rate, and GSH level in the mice with NEC, but the survival rate was not significantly different between NEC and-treated groups (>0.05).

CONCLUSIONS: DSM17938 can offer protection against NEC in mice by reducing oxidative stress and increasing antioxidant capacity of the intestinal tissue to suppress intestinal inflammations.

Print Options

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.