Article Publish Status: FREE
Abstract Title:

Evaluation of Antibacterial, Antineoplastic, and Immunomodulatory Activity of Paullinia cupana Seeds Crude Extract and Ethyl-Acetate Fraction.

Abstract Source:

Evid Based Complement Alternat Med. 2016 ;2016:1203274. Epub 2016 Dec 7. PMID: 28053639

Abstract Author(s):

Lidiane Vasconcelos do Nascimento Carvalho, Marina Ferraz Cordeiro, Thiago Ubiratan Lins E Lins, Maria Clara Pinheiro Duarte Sampaio, Gabriela Souto Vieira de Mello, Valécia de Cassia Mendonça da Costa, Leila Larisa Medeiros Marques, Traudi Klein, João Carlos Palazzo de Mello, Isabella Macário Ferro Cavalcanti, Ivan da Rocha Pitta, Maira Galdino da Rocha Pitta, Moacyr Jesus Barreto de Melo Rêgo

Article Affiliation:

Lidiane Vasconcelos do Nascimento Carvalho


Paullinia cupana (Guarana) is a native plant of Amazon region that has very traditional importance. Its seeds are rich in bioactive compounds, including tannins, which exhibit relevant properties. Objective. This study aimed to evaluate antibacterial, antineoplastic, and immunomodulatory activity of P. cupana seeds crude extract (CE) and ethyl-acetate fraction (EAF). Methods. Antibacterial activity was evaluated by determination of minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). Antineoplastic activity was evaluated by MTT assays in hepatocellular carcinoma (HepG2), breast adenocarcinoma (MCF-7), ductal carcinoma (T47-D), non-Hodgkin's B cell lymphoma (Toledo), T cell leukemia (Jukart), and Acute Leukemia (HL-60) cell lines. BALB/c mice splenocytes were treated to assess IFN-γ, IL-6, IL-17, and IL-10 levels by sandwich ELISA. Results. CE and EAF were not toxic to peripheral blood cells and splenocytes. CE and EAF fractions showed a bacteriostatic activity (MIC = 250 μg/mL) and presented IC50 values of 70.25 μg/mL and 61.18 μg/mL in HL-60 leukemia cell line. All cytokines evaluated had their levels reduced after treatment, following dose-response model. Discussion and Conclusion. Different biological activities were observed for both CE and EAF, suggesting P. cupana as a source of bioactive substances, especially tannins that may be used for several diseases treatments.

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