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Article Publish Status: FREE
Abstract Title:

Eugenol alleviates transmissible gastroenteritis virus-induced intestinal epithelial injury by regulating NF-κB signaling pathway.

Abstract Source:

Front Immunol. 2022 ;13:921613. Epub 2022 Aug 16. PMID: 36052062

Abstract Author(s):

Kang Wang, Daiwen Chen, Bing Yu, Jun He, Xiangbing Mao, Zhiqing Huang, Hui Yan, Aimin Wu, Yuheng Luo, Ping Zheng, Jie Yu, Junqiu Luo

Article Affiliation:

Kang Wang

Abstract:

Increasing evidence supports the ability of eugenol to maintain intestinal barrier integrity and anti-inflammatoryand; however, whether eugenol alleviates virus-mediated intestinal barrier damage and inflammation remains a mystery. Transmissible gastroenteritis virus (TGEV), a coronavirus, is one of the main causative agents of diarrhea in piglets and significantly impacts the global swine industry. Here, we found that eugenol could alleviate TGEV-induced intestinal functional impairment and inflammatory responses in piglets. Our results indicated that eugenol improved feed efficiency in TGEV-infected piglets. Eugenol not only increased serum immunoglobulin concentration () but also significantly decreased serum inflammatory cytokine concentration () in TGEV-infected piglets. In addition, eugenol also significantly decreased the expression ofmRNA and the phosphorylation level ofprotein in the jejunum mucosa of TGEV-infected piglets. Eugenol increased villus height and the ratio of villus height to crypt depth in the jejunum and ileum, and decreased serum D-lactic acid levels. Importantly, eugenol increased tight junction protein () and mRNA expression levels of nutrient transporter-related genes (and) in the jejunum mucosa of TGEV-infected piglets. Meanwhile, compared with TGEV-infected IPEC-J2 cells, treatment with eugenol reduced the cell cytopathic effect, attenuated the inflammatory response. Interestingly, eugenol did not increase the expression ofandin IPEC-J2 cells. However, western blot and immunofluorescence results showed that eugenol restored TGEV-induced down-regulation of ZO-1 and Occludin, while BAY11-7082 (The NF-κB specific inhibitor) enhanced the regulatory ability of eugenol. Our findings demonstrated that eugenol attenuated TGEV-induced intestinal injury by increasing the expression ofand, which may be related to the inhibition ofsignaling pathway. Eugenol may offer some therapeutic opportunities for coronavirus-related diseases.

Study Type : In Vitro Study

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Sayer Ji
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