Abstract Title:

Effects in vitro and in vivo of hesperidin administration in an experimental model of acute lung inflammation.

Abstract Source:

Free Radic Biol Med. 2022 02 20 ;180:253-262. Epub 2022 Jan 26. PMID: 35092853

Abstract Author(s):

Ana Beatriz Farias de Souza, Natália Alves de Matos, Thalles de Freitas Castro, Guilherme de Paula Costa, Laser Antônio Machado Oliveira, Katiane de Oliveira Pinto Coelho Nogueira, Iara Mariana Léllis Ribeiro, André Talvani, Sílvia Dantas Cangussú, Rodrigo Cunha Alvim de Menezes, Frank Silva Bezerra

Article Affiliation:

Ana Beatriz Farias de Souza


Mechanical ventilation (MV) is a tool used in critical patient care. However, it can trigger inflammatory and oxidative processes capable of causing or aggravating lung injuries, which is known as ventilator-induced lung injury (VILI). Hesperidin is a flavonoid with antioxidant and anti-inflammatory properties in various diseases. The role of hesperidin in the process triggered by MV is poorly studied. Thus, we hypothesize hesperidin could protect the lung of mice submitted to mechanical ventilation. For that, we evaluated cell viability and reactive oxygen species (ROS) formation in macrophages using different hesperidin concentrations. We observed hesperidin did not reduce cell viability, however; it attenuated the production of intracellular ROS in cells stimulated with lipopolysaccharide (LPS). We further evaluated the effects of hesperidin in vivo in animals submitted to MV. In the bronchoalveolar lavage fluid, there were higher levels of macrophage, lymphocyte and neutrophil counts in animals submitted to MV, indicating an inflammatory process. In the lung tissue, MV induced oxidative damage and increased myeloperoxidase activity, though the antioxidant enzyme activity decreased. MV also induced the production of the inflammatory mediators CCL-2, TNF-α and IL-12. Pretreatment with hesperidin resulted in less recruitment of inflammatory cells to the airways and less oxidative damage. Also, it reduced the formation of CCL-2 and IL-12. Our results show pretreatment with hesperidin can protect the lungs of mice submitted to mechanical ventilation by modulating the inflammatory response and redox imbalance and may act to prevent MV injury.

Study Type : Animal Study, In Vitro Study
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Additional Keywords : Ventilator Mortality

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Sayer Ji
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