Article Publish Status: FREE
Abstract Title:

Effect ofextract on PI3K/Akt pathway in DMBA induced breast cancer in albino rats.

Abstract Source:

Biosci Rep. 2018 Aug 20. Epub 2018 Aug 20. PMID: 30126855

Abstract Author(s):

Mohamed Abdo Nassan, Mohamed Mohamed Soliman, Shimaa Ahmed Ismail, Samir Ahmed El-Shazly

Article Affiliation:

Mohamed Abdo Nassan


BACKGROUND: Breast cancer is one of the most prevalent types of cancer and a leading cause of death in women.

MATERIALS AND METHODS: An experimental model of breast cancer was induced in female albino rats using single intragastric dose of 7, 12 dimethylbenz (α) anthracene (DMBA) in sesame oil (50 mg/kg b.wt). Four months after DMBA administration, incidence of breast cancer was confirmed by measuring cancer antigen 15-3 (CA15-3) serum levels.root extract (TOE) was administered in a dose of 500 mg/kg by oral gavage for 4 weeks after breast cancer incidence. Level of CA15-3 as one of the best known breast tumor markers, was elevated in all positive breast cancer rats. The genetic effects of TOE on Pdk1- Akt1- Pik3r1- Map3k1- Erbb2- PIk3ca using semiquantitative RT-PCR analysis were evaluated. In parallel, histopathological changes and immunohistochemical expression of Bcl2 in mammary gland tissues was examined.

RESULTS: level of CA15-3 was normalized in DMBA group administered TOE extract for 4 weeks. Administration of DMBA increased expression of Pdk1, Akt1, Pik3r1, Map3k1, Erbb2 and PIk3ca. Treatment with TOE normalized the up-regulated mRNA for all examined genes except Pik3ra that was up-regulated. Mammary gland tissues of DMBA group showed excessive proliferation of lining epithelium of acini and ductules with hyperchromatic nuclei with excessive immunostaing of Bcl2 in proliferated epithelium that was ameliorated by TOE administration. In conclusion TOE regulated PI3K and Akt pathways involved in suppression of breast cancer growth and proliferation. TOE is effective as anticancer herbal agent.

Study Type : Animal Study

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