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Article Publish Status: FREE
Abstract Title:

Chondroprotective Effect of Cynaroside in IL-1-Induced Primary Rat Chondrocytes and Organ Explants via NF-B and MAPK Signaling Inhibition.

Abstract Source:

Oxid Med Cell Longev. 2020 ;2020:9358080. Epub 2020 Jan 24. PMID: 32047580

Abstract Author(s):

Seul Ah Lee, Bo-Ram Park, Sung-Min Moon, Joon Ho Hong, Do Kyung Kim, Chun Sung Kim

Article Affiliation:

Seul Ah Lee

Abstract:

Osteoarthritis (OA) is a degenerative joint disease characterized by cartilage degradation and inflammation. Interleukin-1is the key player in the pathogenesis of OA, which induces the expression of various catabolic factors that contribute to cartilage degradation. Cynaroside (luteolin-7-O-glucoside or luteoloside) is a flavonoid that has various pharmacological properties, such as antitumor, anti-inflammatory, and antioxidant activities. In this study, we investigated the chondroprotective effects of cynaroside on IL-1-stimulated chondrocytes and organ explants. The production of nitrite, PGE, collagen type II, and aggrecan was measured by a Griess reagent and ELISAs, and the production of ROS was measured by HDCF-DA fluorescence. The protein levels of iNOS, Cox-2, MMP-1, MMP-3, MMP-13, ADAMTS-4, MAPKs, and the NF-B p65 subunit were measured by western blot. Proteoglycan analysis was performed by Alcian Blue staining () and Safranin O staining (). Cynaroside inhibited IL-1-induced expression of catabolic factors (nitrite, iNOS, ROS, PGE, Cox-2, MMP-1, MMP-3, MMP-13, and ADAMTS-4) and degradation of anabolic factors (collagen type II and aggrecan). Furthermore, cynaroside suppressed IL-1-induced phosphorylation of MAPKs and translocation of the NF-B p65 subunit into the nucleus. Collectively, these results suggest that cynaroside may be a potential candidate for the development of new therapeutic drugs for the alleviation of OA progression.

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