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Abstract Title:

Antimicrobial Activity ofLeaf Extracts againstf. sp.and Analysis of Its Fractions by HPLC.

Abstract Source:

Pathogens. 2022 Mar 12 ;11(3). Epub 2022 Mar 12. PMID: 35335671

Abstract Author(s):

Qurat Ul Ain, Shahzad Asad, Karam Ahad, Muhammad Naeem Safdar, Atif Jamal

Article Affiliation:

Qurat Ul Ain

Abstract:

Fusarium wilt has ruined banana production and poses a major threat to its industry because of highly virulentf. sp.(Foc) race 4. The present study focused on the efficacy ofleaf extracts and its organic fractions against Foc in in vitro and greenhouse experiments. The presence of polyphenols in the fractions was also investigated using high performance liquid chromatography (HPLC). The in vitro tests carried out for the leaf extract ofshowed its inhibitory effect on the mycelial growth and, based on this evidence, further characterization of fractions were done. Complete mycelial inhibition and the highest zone of inhibition against Foc was observed for the n-butanol fraction in vitro, while the n-hexane and dichloromethane fractions showed lower disease severity index (DSI) in greenhouse experiments. The fractions were further analysed by HPLC using nine polyphenolic standards, namely quercitin, myrecitin, kaempferol, rutin, gallic acid, trans-ferulic acid, coumeric acid, epicatechin and catechin. The highest content of polyphenols, based on standards used, was quantified in the n-butanol fraction followed by the ethyl acetate fraction of the leaf extract. This is the first report of antimicrobial activity ofextracts against Foc to the best of our knowledge.

Study Type : In Vitro Study

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