Abstract Title:

Allicin inhibits proliferation and promotes apoptosis of human epidural scar fibroblasts.

Abstract Source:

World Neurosurg. 2020 Jan 14. Epub 2020 Jan 14. PMID: 31953094

Abstract Author(s):

Hui-Hui Sun, Jing-Cheng Wang, Xin-Min Feng, Shou-Lei Zhu, Jun Cai

Article Affiliation:

Hui-Hui Sun


BACKGROUND: Allicin can suppress liver and cardiac fibrosis, thus we hypothesize that it may prevent scar tissue from extensive epidural fibrosis after laminectomy.

METHODS: Human epidural scar fibroblasts were isolated from surgical specimen, and treated by allicin at a gradient of concentrations. The morphology, viability, migration rate, cell cycle and apoptosis rate were measured by fluorescence microscope, CCK-8 assay, scratch assay and flow cytometry. Western Blot was used to measure the expression level of proliferation-related proteins.

RESULTS: After treated by allicin, cell viability (p=0.042*) and migration rate (p=0.010* in scratch assay, and p=0.025* in Transwell assay) decreased significantly in a dose-dependent manner. The percentage of G1 phase cells significantly decreased (p=0.017*), while that of S phase (p=0.096*) and G2 (p=0.038*) phase cells significantly increased in a dose-dependent manner. Similarly, the percentage of apoptotic cells increased significantly in a dose-dependent manner (p=0.036*). Comparing with control group, the expression level of PCNA protein (p=0.081) and Bcl-2 protein (p=0.029*) significantly decreased, while the Bax protein level significantly increased (p=0.017*).

CONCLUSION: Allicin can suppress human epidural scar fibroblasts migration, induce cell apoptosis, and block cell proliferation at S phase and G2 phase.

Study Type : In Vitro Study

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.